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GFP antibody - 132 003AT1

GFP is a widely used green fluorescent protein tag
Polyclonal rabbit purified antibody
Cat. No.: 132 003AT1
Amount: 50 µg
Price: $475.00
Cat. No. 132 003AT1 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with ATTO® 647N

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established CyDye fluorescence detection systems can be used.
ATTO® 550 has similar exitation and emission properties as Cy3, and ATTO® 647N as Cy5.
ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®594 is also recommended for PALM and dSTORM high resolution microscopy.

ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
CyDyes, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Label ATTO 647N
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_003at1.pdf
Cat. No.: 132 003AT1
Quantity: 50 µg
Price: $475.00
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.