Cat. No.: 710 006DY1
Amount: 5 nmol
Price:
$355.00
|
|
|
| Cat. No. 710 006DY1 |
5nmol mCLING labeled with DY® 654 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use. |
| Applications |
IHC: 1 : 25 up to 1 : 50 (2 - 1 nmol/ml) |
| Label | DY 654 |
| Remarks |
Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016. |
| Data sheet | 710_006dy1.pdf |
|
|