mCLING-DY 654-labeled - 710 006DY1
A fixable membrane probe for high resolution microscopy
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|Cat. No. 710 006DY1||
5nmol mCLING labeled with DY® 654 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.