Synaptic Systems - Abeta-pE3

Cat. No.: 218 108

Amount: 50 µg

Price: $420.00

Abeta-pE3-staining of plaques (red) in a transgenic APP/PS1 Alzheimer’s disease mouse brain section

Dot blot

IHC

IHC-P

Background

Amyloid deposits, also called plaques, of Alzheimer's patients consist of several protein components like the amyloid beta-peptides (Abeta, Aβ) 1-40/42 and additional C- and N-terminally truncated and modified fragments. Very abundant are the isoaspartate (isoAsp)-Abeta and pyroglutamyl (pGlu)-Abeta peptides. The latter are formed by cyclization of the N-terminal glutamate at position 3 or 11 catalyzed by glutaminyl cyclase (QC) resulting in very amyloidogenic and neurotoxic variants of Abeta; Abeta-pE3 and Abeta pE11.
In contrast to extracellular plaques that do not perfectly correlate with Alzheimer´s disease intraneuronal Abeta accumulation and vascular Abeta deposits have gained more and more evidence to be among the crucial factors responsible for progressive neuron loss.

1 ) Sullivan CP et al., Neurosci. Lett. (2011)
2 ) Perez-Garmendia R et al., J. Neuroimmunol. (2010)
3 ) Schilling S et al., Nat. Med. (2008)
4 ) Hook V et al., Biol. Chem. (2008)
5 ) Böhme L et al., Biol. Chem. (2008)
6 ) Schilling S et al., J. Neurochem. (2008)
7 ) Wiltfang J et al., J. Biol. Chem. (2001)
8 ) Wood SJ et al., J. Mol. Biol. (1996)
9 ) Kuo YM et al., J. Biol. Chem. (1996)

Protocols

IHC

IHC-P

Other products for this target

Cat. No. 218 108	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet Dot blot: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 5000 (see remarks) gallery IHC-P: 1 : 1000 up to 1 : 4000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunodetection of target molecules spotted onto a membrane. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb2-48
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to the amino terminal part of human Abeta-pE3 (UniProt Id: P05067)
Epitop	AA 3 to 7 from human Abeta-pE3 (UniProt Id: P05067)
Reactivity	Reacts with: human (P05067), mouse (P12023), rat (P08592), monkey. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 2-48. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. Dot blot: This application was only tested with synthetic peptides. Please boil membrane for 3 minutes in PBS before blocking and antibody incubation. IHC: Antigen retrieval with formic acid is required. IHC-P: Antigen retrieval with citrate buffer pH 6, followed by formic acid treatment, is required for chromogenic detection.
Data sheet	Datasheet 218_108

Abeta-pE3 antibody - 218 108