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SY SY - Synaptic Systems SY SY - Synaptic Systems

mCLING-ATTO 488-labeled - 710 006AT3

A fixable membrane probe for high resolution microscopy

330.00 USD
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Cat. No. 710 006AT3 5nmol mCLING labeled with ATTO® 488 in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.
Applications ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Label ATTO 488
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Datasheet 710_006at3.pdf


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References for mCLING-ATTO 488-labeled - 710 006AT3

A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
Visualization of Membrane Pore in Live Cells Reveals a Dynamic-Pore Theory Governing Fusion and Endocytosis.
Shin W, Ge L, Arpino G, Villarreal SA, Hamid E, Liu H, Zhao WD, Wen PJ, Chiang HC, Wu LG
Cell (2018) : . 710 006AT3 ICC
A fixable probe for visualizing flagella and plasma membranes of the African trypanosome.
Wiedeman J, Mensa-Wilmot K
PloS one (2018) 135: e0197541. 710 006AT3 ICC, FACS
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com