print page Visit us on Instagram Visit us on Facebook Visit us on Twitter Visit us on LinkedIn
SY SY - Synaptic Systems SY SY - Synaptic Systems

mCLING-ATTO 647N-labeled - 710 006AT1

A fixable membrane probe for high resolution microscopy

330.00 USD
select country for shop
Cat. No. 710 006AT1 5nmol mCLING labeled with ATTO® 647N in 100 µl PBS (lyophilized). For reconstitution add 100 µl H2O, then aliquot and store at -80°C until use.
Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye.
Applications ICC: 1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml) gallery  
IHC: 1 : 25 up to 1 : 50 (1 - 2 nmol/ml)
Label ATTO 647N
Remarks

Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.

Datasheet 710_006at1.pdf


For online shopping select your country:
If your country is not listed, please contact us by email to sales@sysy.com or contact your local distributor.




References for mCLING-ATTO 647N-labeled - 710 006AT1

Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.
Baer A, Hänsch S, Mayer G, Harrington MJ, Schmidt S
Biomacromolecules (2018) : . 710 006AT1 ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA, Lind AL, Smith SE, Li R, Gould KL
eLife (2017) 6: . 710 006AT1 ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.
Taniguchi K, Shao Y, Townshend RF, Cortez CL, Harris CE, Meshinchi S, Kalantry S, Fu J, O'Shea KS, Gumucio DL
The Journal of cell biology (2017) 21612: 3981-3990. 710 006AT1 ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.
Baer A, Schmidt S, Haensch S, Eder M, Mayer G, Harrington MJ
Nature communications (2017) 81: 974. 710 006AT1 ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Baranov MV, Revelo NH, Dingjan I, Maraspini R, Ter Beest M, Honigmann A, van den Bogaart G
Cell reports (2016) 176: 1518-1531. 710 006AT1 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.
Reuveny A, Shnayder M, Lorber D, Wang S, Volk T
Development (Cambridge, England) (2018) 14517: . 710 006AT1 IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.
Chartier TF, Deschamps J, Dürichen W, Jékely G, Arendt D
Open biology (2018) 810: . 710 006AT1 IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.
Revelo NH, Rizzoli SO
Current protocols in neuroscience (2016) 74: 2.25.1-21. 710 006AT1 ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.
Jung S, Maritzen T, Wichmann C, Jing Z, Neef A, Revelo NH, Al-Moyed H, Meese S, Wojcik SM, Panou I, Bulut H, et al.
The EMBO journal (2015) 3421: 2686-702. 710 006AT1 IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.
Revelo NH, Kamin D, Truckenbrodt S, Wong AB, Reuter-Jessen K, Reisinger E, Moser T, Rizzoli SO
The Journal of cell biology (2014) 2054: 591-606. 710 006AT1 ICC, IHC
Synaptic Systems Gesellschaft für neurobiologische Forschung, Entwicklung und Produktion mbH | Rudolf-Wissell-Str. 28 | 37079 Goettingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com | Impressum - Legal Note | Privacy Policy
Design by BLACKBIT interactive GmbH

Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com