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PSD95 sdAb - FluoTag-X2 - N3702-At488-L

PSD 95 is a component of postsynaptic densities in central synapses

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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3702-At488-L
Amount: 200 µl
Price: $485.00
Cat. No. N3702-At488-L

2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage –80°C for up to 12 month
Applications
 
WB: N/A
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1B2
Subtype single domain
Immunogen Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)
Reactivity Reacts with: mouse (Q62108), rat (P31016).
Other species not tested yet.
Specificity Specific for PSD 95
Matching control protein/peptide 124-01P
Documents data sheet

Cat. No.: N3702-At488-L
Amount: 200 µl
Price: $485.00
Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.