This product was developed by
Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N3102-At488-L||
2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
|Storage||–80°C for up to 12 month|
WB: not recommended
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Label||ATTO 488, two fluorophores coupled to one FluoTag|
|Specificity||Specific for mEOS and mEOS derivatives|
mEOS, is a bright monomeric green photoswitchable fluorescent protein derived from Lobophyllia hemprichii.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.