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Home > Products > N1502-At488-L

ALFA sdAb - FluoTag-X2 - N1502-At488-L

The ALFA tag is a novel peptide tag

 

This product was developed by   

 NanoTag-Biotechnologies

Camelid single domain antibodies consist only of one antigen binding site of a Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules. FluoTags carry fluorophore molecules coupled in a site specific manner.

 

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.

Camelid single domain antibody

Cat. No.: N1502-At488-L
Amount: 200 µl
Price: $515.00
Cat. No. N1502-At488-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488.

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established fluorescence detection systems can be used.
ATTO® 488: λex 500 nm / λem 520 nm
ATTO® 550: λex 554 nm / λem 576 nm
ATTO® 647N: λex 646 nm / λem 664 nm

ATTO®488, ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®488 and ATTO®594 are also recommended for PALM and dSTORM high resolution microscopy.

This product or portions thereof is manufactured under license from ATTO-TEC GmbH.
ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation.

 Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect from light!
Applications
 
ICC: 1 : 1000
IHC: 1 : 1000
IHC-P: 1 : 1000
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1G5
Subtype single domain
Immunogen Synthetic peptide corresponding to: SRLEEELRRRLTE (UniProt Id: not defined)
Specificity Recognizes the ALFA tag: SRLEEELRRRLTE

References for ALFA sdAb - N1502-At488-L

Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
bioRxiv : the preprint server for biology (2023) : . N1502-At488-L IHC; tested species: drosophila

Cat. No.: N1502-At488-L
Amount: 200 µl
Price: $515.00
Mapping of multiple neurotransmitter receptor subtypes and distinct protein complexes to the connectome.
Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, et al.
bioRxiv : the preprint server for biology (2023) : . N1502-At488-L IHC; tested species: drosophila
Background

The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts.
The ALFA-tag has the broadest spectrum of applications in life sciences compared to any other tag. NanoTag developed a nanobody binding the ALFA-tag with ~26 pM affinity, which is ideally suited not only for super-resolution microscopy, immunoprecipitations and Western blotting, but also allows detection of proteins within living cells. This nanobody can be conjugated to virtually all kinds of fluorophores, enyzmes or other biomolecules.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

 
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