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Gut lysate - 606-GUT

Ready to use tissue lysates for western blots
Rat tissue lysate
Cat. No.: 606-GUT
Amount: 200 µg
Price: $90.00
Cat. No. 606-GUT 200 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'.
Total volume: 100 µl. Concentration: 2 mg/ml.
Aliquot and store at -20°C until use.
WB: yes gallery  
Loading/lane 10-30 µg
Source Rat, White Wistar

Since several proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.

Data sheet 606-gut.pdf
Documents MSDS
Cat. No.: 606-GUT
Amount: 200 µg
Price: $90.00

In many cases the relative concentration of the protein of interest is high enough to use whole tissue lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Protein concentration has been determined with a Bradford protein quantification assay.