|Cat. No. 135-4P||
100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
|Immunogen||Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)|
|Recommended dilution||Optimal concentrations should be determined by the end-user.|
|Matching antibodies||135 402, 135 403, 135 404, 135 416, 135 421, 135 408, 135 418, 135 421BT, 135 421C3, 135 421C5, 135 409|
This control protein consists of the Strep-Tag® fusion protein (aa 510 - 582 of rat VGLUT 2) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.
The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.