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VGLUT2 antibody - 135 421C3

VGluT2 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 421C3
Amount: 100 µg
Price: $470.00
Cat. No. 135 421C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 95E11
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity Reacts with: mouse (Q8BLE7), rat (Q9JI12).
Other species not tested yet.
Matching control protein/peptide 135-4P
Data sheet 135_421c3.pdf
Cat. No.: 135 421C3
Amount: 100 µg
Price: $470.00

The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.