Synaptic Systems

Cat. No.: 135 416

Amount: 50 µg

Price: $390.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 135 416	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 5000 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT2 (UniProt Id: Q9JI12)
Reactivity	Reacts with: rat (Q62634), mouse (Q3TXX4). Other species not tested yet.
Matching control protein/peptide	135-4P
Remarks	This antibody is highly recommended as a marker for glutamatergic nerve terminals. WB: To avoid protein aggregation, do not heat samples for SDS-PAGE.
Data sheet	Datasheet 135_416

References for VGLUT2 - 135 416

Macrophage-glia interactions regulate immune damage to enteric neurons during West Nile virus infection.
Janova H, Zhao FR, Akgul A, Schatz M, Alligood DM, Alvarado DM, Thackray LB, Stappenbeck TS, Diamond MS
Proceedings of the National Academy of Sciences of the United States of America (2026) 12311: e2537058123. 135 416 IHC; tested species: mouse

Selective regulation of corticostriatal synapses by astrocytic phagocytosis.
Kim JY, Kim H, Chung WS, Park H
Nature communications (2025) 161: 2504. 135 416 IHC; tested species: mouse

The effects of amyloidosis and aging on glutamatergic and GABAergic synapses, and interneurons in the barrel cortex and non-neocortical brain regions.
Qu T
Frontiers in neuroanatomy (2025) 19: 1526962. 135 416 IHC; tested species: mouse

Identification of a neural basis for energy expenditure in the mouse arcuate hypothalamus.
Wang T, Han S, Wang Y, Li Y, Li Y, Xing Z, Zhang S, Xia Y, Zhu F, Chen ZH, Li YZ, et al.
Neuron (2025) 11322: 3813-3833.e9. 135 416 IHC; tested species: mouse

Behavioral decline in Shank3Δex4-22 mice during early adulthood parallels cerebellar granule cell glutamatergic synaptic changes.
Kshetri R, Beavers JO, Hyde R, Ewa R, Schwertman A, Porcayo S, Richardson BD
Molecular autism (2024) 151: 52. 135 416 IHC; tested species: mouse

Synaptic circuits involving gastrin-releasing peptide receptor-expressing neurons in the dorsal horn of the mouse spinal cord.
Quillet R, Gutierrez-Mecinas M, Polgár E, Dickie AC, Boyle KA, Watanabe M, Todd AJ
Frontiers in molecular neuroscience (2023) 16: 1294994. 135 416 IHC; tested species: mouse

Glutamate delta 1 receptor regulates autophagy mechanisms and affects excitatory synapse maturation in the somatosensory cortex.
Gawande DY, S Narasimhan KK, Bhatt JM, Pavuluri R, Kesherwani V, Suryavanshi PS, Shelkar GP, Dravid SM
Pharmacological research (2022) 178: 106144. 135 416 IHC; tested species: mouse

Calretinin-Expressing Synapses Show Improved Synaptic Efficacy with Reduced Asynchronous Release during High-Rate Activity.
Zhang C, Wang M, Lin S, Xie R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4213: 2729-2742. 135 416 IHC; tested species: mouse

Local targets of T-stellate cells in the ventral cochlear nucleus.
Lin L, Campbell J, Oertel D, Smith PH
The Journal of comparative neurology (2022) : . 135 416 IHC; tested species: mouse

Antibodies Against the Gastrin-releasing Peptide Precursor Pro-Gastrin-releasing Peptide Reveal Its Expression in the Mouse Spinal Dorsal Horn.
Gutierrez-Mecinas M, Kókai É, Polgár E, Quillet R, Titterton HF, Weir GA, Watanabe M, Todd AJ
Neuroscience (2022) 510: 60-71. 135 416 IHC; tested species: mouse

EASI-FISH for thick tissue defines lateral hypothalamus spatio-molecular organization.
Wang Y, Eddison M, Fleishman G, Weigert M, Xu S, Wang T, Rokicki K, Goina C, Henry FE, Lemire AL, Schmidt U, et al.
Cell (2021) : . 135 416 IHC; tested species: mouse

Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb.
Naik AS, Lin JM, Taroc EZM, Katreddi RR, Frias JA, Lemus AA, Sammons MA, Forni PE
Development (Cambridge, England) (2020) 1478: . 135 416 IHC; tested species: mouse

Optogenetic "low-theta" pacing of the septohippocampal circuit is sufficient for spatial goal finding and is influenced by behavioral state and cognitive demand.
Mouchati PR, Kloc ML, Holmes GL, White SL, Barry JM
Hippocampus (2020) 3011: 1167-1193. 135 416 IHC; tested species: rat

Circuit dissection of the role of somatostatin in itch and pain.
Huang J, Polgár E, Solinski HJ, Mishra SK, Tseng PY, Iwagaki N, Boyle KA, Dickie AC, Kriegbaum MC, Wildner H, Zeilhofer HU, et al.
Nature neuroscience (2018) : . 135 416 IHC; tested species: mouse

Cat. No.: 135 416

Amount: 50 µg

Price: $390.00

Selective regulation of corticostriatal synapses by astrocytic phagocytosis.
Kim JY, Kim H, Chung WS, Park H
Nature communications (2025) 161: 2504. 135 416 IHC; tested species: mouse

Local targets of T-stellate cells in the ventral cochlear nucleus.
Lin L, Campbell J, Oertel D, Smith PH
The Journal of comparative neurology (2022) : . 135 416 IHC; tested species: mouse

Background

The vesicular glutamate transporter 2 VGLUT2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT1. Like VGLUT1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT1 and VGLUT2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Takamori S et al., J. Neurosci. (2001)
2 ) Fremeau RT et al., Neuron (2001)
3 ) Fujiyama F et al., J. Comp. Neurol. (2001)
4 ) Aihara Y et al., J. Neurochem. (2000)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

VGLUT2 (SLC17A6) antibody - 135 416

References for VGLUT2 - 135 416