|Cat. No. 135 421C5||
100 µg purified IgG, lyophilized, fluorescence-labeled with Sulfo-Cyanine 5. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: not recommended (see remarks)
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG2a (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 566 to 582 from rat VGLUT2 (UniProt Id: Q9JI12)|
Reacts with: rat (Q9JI12), mouse (Q8BLE7).
Other species not tested yet.
|Specificity||Specific for VGLUT 2|
ICC: As a Sulfo-Cyanine 5 conjugate this antibody produces some unspecifc nuclear staining in cultured primary neurons.
The vesicular glutamate transporter 2 VGLUT 2, also referred to as DNPI and SLC17A6, has a more restricted expression than the related VGLUT 1. Like VGLUT 1, it is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.