Cat. No.: 302-21P
Amount: 100 µg
Price:
$110.00
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| Cat. No. 302-21P |
100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Control peptides should be stored at -20°C when still lyophilized! |
| Applications |
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| Immunogen | Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366) |
| Recommended dilution | Optimal concentrations should be determined by the end-user. |
| Matching antibodies | 302 204, 302 211, 302 211C3, 302 411, 302 206, 302 308, 302 209 |
| Remarks |
This control peptide consists of the synthetic peptide SEAREDMAALEKDYEEV (aa 419 - 435 in human α-tubulin 4A) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen. |
| Data sheet | 302-21p.pdf |
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Microtubules are involved in a wide variety of intracellular events including cell division, intracellular transport and secretion, axonal transport, and maintenance of cell morphology. They are composed of tubulin, a heterodimeric protein, consisting of two polypeptides, α-tubulin and β-tubulin (1).
α Tubulin undergoes numerous post-translational modifications that include tyrosination-detyrosination and deglutamylation, phosphorylation, acetylation, polyglutamylation, and polyglycylation. In one of the major posttranslational modifications, the C-terminal tyrosine residue in α-tubulin is added or removed reversibly, producing Glu-tubulin (after detyrosination) and Tyr-tubulin (with re-added tyrosine). Early stages of cell development are often enriched in Tyr tubulin, whereas mature cells show increased Glu tubulin in stable structures. Some microtubule associated proteins (MAPs), motor proteins like kinesins, or stabilizing factors have different affinities for Glu- or Tyr-tubulin (2,3,4).
A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by tyrosine ligase and is one of the dominant α-tubulin isoforms in neurons (5).