|Cat. No. 302 211||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 500 up to 1 : 1000 gallery
ELISA: yes (see remarks)
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)|
Epitop: AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Reacts with: human (P68366), rat, mouse, vertebrates, invertebrates, yeast.
Other species not tested yet.
|Specificity||Specific for α-tubulin (glu- and tyr-α-tubulin)|
|Matching control protein/peptide||302-21P|
ELISA: Suitable as capture antibodies for sandwich-ELISA with cat. no. 302 203 as detector antibody.
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.
Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.