Tailor-made Antibodies
and Tools for Life Science
Home|||||||Technical Support|

alpha-Tubulin antibody - 302 411

alpha-Tubulin is a major cytoskeleton protein
Mouse monoclonal purified IgM
Cat. No.: 302 411
Amount: 100 µg
Price: $415.00
Cat. No. 302 411 100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Clone 88B6
Subtype IgM (κ light chain)
Immunogen Synthetic peptide corresponding to AA 419 to 435 from human α-tubulin 4A (UniProt Id: P68366)
Epitop Epitop: AA 419 to 485 from human α-tubulin 4A (UniProt Id: P68366)
Reactivity Reacts with: human (P68366), rat (Q5XIF6), mouse (P68368).
Other species not tested yet.
Specificity Specific for α-tubulin.
Matching control protein/peptide 302-21P
Data sheet 302_411.pdf
Cat. No.: 302 411
Amount: 100 µg
Price: $415.00
Background

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
Tubulin itself is a globular protein which consists of two polypeptides, α-tubulin and β-tubulin. α- and β-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter.

Assembled microtubules can be detyrosinated by a carboxypeptidaseS called vasohibins / SVBPs. Detyrosinated α-tubulin is referred to as Glu-α-tubulin and occurs for exemple in neurons. This reaction can be reverted by Tubulin tyrosine ligase (TTL) that ads a C-terminal tyrosin to Glu α-tubulin.
Another post-translational modification of α-tubulin is C-terminal polyglutamylation which is also characteristic for microtubules in neuronal cells and the mitotic spindle. A third variant of detyrosinated α-tubulin is Δ2-tubulin which lacks the C-terminal glutamic acid. It cannot be tyrosinated by TTL and is one of the dominant α-tubulin isoforms in neurons.