GFAP - 173 011
A glial-specific type-III intermediate filament protein
Monoclonal mouse purified IgG
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|Cat. No. 173 011||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
ICC: 1 : 500 gallery
IHC: 1 : 200 gallery
IHC-P/FFPE: 1 : 500 gallery
ELISA: yes (see remarks)
|Subtype||IgG2a (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)|
Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for GFAP isoform 1.|
|matching control protein/peptide||173-0P|
WB: The monoclonal antibodies are less sensitive compared to the polyclonals.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 173 002 or 173 211BT as detector antibody (protocol for sandwich-ELISA).