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GFAP - 173 002

A glial-specific type-III intermediate filament protein
Polyclonal rabbit antiserum
Cat. No.: 173 002
Amount: 200 µl
Price: $350.00
Cat. No. 173 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: yes gallery  
IHC-P/FFPE: 1 : 1000 gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), chicken, zebrafish.
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms.
Matching control protein/peptide 173-0P
Remarks

WB: The polyclonal antibodies are more sensitive compared to the monoclonals.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 173 011 as capture antibody.

Data sheet 173_002.pdf

References for GFAP - 173 002

Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 173 002 WB; tested species: rat
Distinct in vivo roles of secreted APP ectodomain variants APPsα and APPsβ in regulation of spine density, synaptic plasticity, and cognition.
Richter MC, Ludewig S, Winschel A, Abel T, Bold C, Salzburger LR, Klein S, Han K, Weyer SW, Fritz AK, Laube B, et al.
The EMBO journal (2018) : . 173 002 WB, IHC; tested species: mouse
Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 173 002 WB
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 173 002 ICC, IHC; tested species: mouse
Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses.
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee CE, Happel MFK, Gundelfinger E, Dityatev A, Frischknecht R, Seidenbecher C
Cells (2020) 92: . 173 002 ICC; tested species: rat
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 173 002 ICC; tested species: rat
Biallelic Mutations in TSC2 Lead to Abnormalities Associated with Cortical Tubers in Human iPSC-Derived Neurons.
Winden KD, Sundberg M, Yang C, Wafa SMA, Dwyer S, Chen PF, Buttermore ED, Sahin M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3947: 9294-9305. 173 002 ICC; tested species: human
Glia-to-neuron transfer of miRNAs via extracellular vesicles: a new mechanism underlying inflammation-induced synaptic alterations.
Prada I, Gabrielli M, Turola E, Iorio A, D'Arrigo G, Parolisi R, De Luca M, Pacifici M, Bastoni M, Lombardi M, Legname G, et al.
Acta neuropathologica (2018) 1354: 529-550. 173 002 ICC; tested species: mouse
Long-term culture of astrocytes attenuates the readily releasable pool of synaptic vesicles.
Kawano H, Katsurabayashi S, Kakazu Y, Yamashita Y, Kubo N, Kubo M, Okuda H, Takasaki K, Kubota K, Mishima K, Fujiwara M, et al.
PloS one (2012) 710: e48034. 173 002 ICC
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 173 002 ICC, IHC; tested species: mouse
Loss of all three APP family members during development impairs synaptic function and plasticity, disrupts learning, and causes an autism-like phenotype.
Steubler V, Erdinger S, Back MK, Ludewig S, Fässler D, Richter M, Han K, Slomianka L, Amrein I, von Engelhardt J, Wolfer DP, et al.
The EMBO journal (2021) : e107471. 173 002 IHC; tested species: mouse
Glial activation is moderated by sex in response to amyloidosis but not to tau pathology in mouse models of neurodegenerative diseases.
Biechele G, Franzmeier N, Blume T, Ewers M, Luque JM, Eckenweber F, Sacher C, Beyer L, Ruch-Rubinstein F, Lindner S, Gildehaus FJ, et al.
Journal of neuroinflammation (2020) 171: 374. 173 002 IHC; tested species: mouse
Distinct in vivo roles of secreted APP ectodomain variants APPsα and APPsβ in regulation of spine density, synaptic plasticity, and cognition.
Richter MC, Ludewig S, Winschel A, Abel T, Bold C, Salzburger LR, Klein S, Han K, Weyer SW, Fritz AK, Laube B, et al.
The EMBO journal (2018) : . 173 002 WB, IHC; tested species: mouse
Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration.
Plaas M, Seppa K, Reimets R, Jagomäe T, Toots M, Koppel T, Vallisoo T, Nigul M, Heinla I, Meier R, Kaasik A, et al.
Scientific reports (2017) 71: 10220. 173 002 IHC; tested species: rat
An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings.
Matsumura R, Yamamoto H, Niwano M, Hirano-Iwata A
Applied physics letters (2016) 1082: 023701. 173 002 IHC
Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies.
Esposito L, Drexler JF, Braganza O, Doberentz E, Grote A, Widman G, Drosten C, Eis-Hübinger AM, Schoch S, Elger CE, Becker AJ, et al.
Epilepsia (2015) 562: 234-43. 173 002 IHC; tested species: human
βCTF-correlated burst of hippocampal TNFα occurs at a very early, pre-plaque stage in the TgCRND8 mouse model of Alzheimer's disease.
Cavanagh C, Colby-Milley J, Bouvier D, Farso M, Chabot JG, Quirion R, Krantic S
Journal of Alzheimer's disease : JAD (2013) 362: 233-8. 173 002 IHC
Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus.
Araya-Callís C, Hiemke C, Abumaria N, Flugge G
Psychopharmacology (2012) 2241: 209-22. 173 002 IHC
Age-dependent kinetics of dentate gyrus neurogenesis in the absence of cyclin D2.
Ansorg A, Witte OW, Urbach A
BMC neuroscience (2012) 13: 46. 173 002 IHC
No improvement after chronic ibuprofen treatment in the 5XFAD mouse model of Alzheimer's disease.
Hillmann A, Hahn S, Schilling S, Hoffmann T, Demuth HU, Bulic B, Schneider-Axmann T, Bayer TA, Weggen S, Wirths O
Neurobiology of aging (2012) 334: 833.e39-50. 173 002 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 173 002 IHC
Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys.
Ribic A, Zhang M, Schlumbohm C, Mätz-Rensing K, Uchanska-Ziegler B, Flügge G, Zhang W, Walter L, Fuchs E
Cellular and molecular neurobiology (2010) 306: 827-39. 173 002 IHC
Coping with AIDS: issues of accountability in the dental office.
Cutler LS
Journal - Connecticut State Dental Association (1988) 621: 20-2. 173 002 IHC; tested species: mouse
Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease.
Hüttenrauch M, Baches S, Gerth J, Bayer TA, Weggen S, Wirths O
Journal of Alzheimer's disease : JAD (2015) 444: 1291-302. 173 002 IHC-P; tested species: mouse
Accelerated tau pathology with synaptic and neuronal loss in a novel triple transgenic mouse model of Alzheimer's disease.
Saul A, Sprenger F, Bayer TA, Wirths O
Neurobiology of aging (2013) 3411: 2564-73. 173 002 IHC-P; tested species: mouse
Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis.
Wirths O, Erck C, Martens H, Harmeier A, Geumann C, Jawhar S, Kumar S, Multhaup G, Walter J, Ingelsson M, Degerman-Gunnarsson M, et al.
The Journal of biological chemistry (2010) 28553: 41517-24. 173 002 IHC-P; tested species: mouse
Accumulation of intraneuronal Abeta correlates with ApoE4 genotype.
Christensen DZ, Schneider-Axmann T, Lucassen PJ, Bayer TA, Wirths O
Acta neuropathologica (2010) 1195: 555-66. 173 002 IHC-P; tested species: human
The Anti-amyloid Compound DO1 Decreases Plaque Pathology and Neuroinflammation-Related Expression Changes in 5xFAD Transgenic Mice.
Boeddrich A, Babila JT, Wiglenda T, Diez L, Jacob M, Nietfeld W, Huska MR, Haenig C, Groenke N, Buntru A, Blanc E, et al.
Cell chemical biology (2018) : . 173 002 ELISA; tested species: mouse
Cat. No.: 173 002
Quantity: 200 µl
Price: $350.00
Sonic hedgehog expression in the postnatal brain.
Rivell A, Petralia RS, Wang YX, Clawson E, Moehl K, Mattson MP, Yao PJ
Biology open (2019) : . 173 002 WB; tested species: rat
Distinct in vivo roles of secreted APP ectodomain variants APPsα and APPsβ in regulation of spine density, synaptic plasticity, and cognition.
Richter MC, Ludewig S, Winschel A, Abel T, Bold C, Salzburger LR, Klein S, Han K, Weyer SW, Fritz AK, Laube B, et al.
The EMBO journal (2018) : . 173 002 WB, IHC; tested species: mouse
Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 173 002 WB
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 173 002 ICC, IHC; tested species: mouse
Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses.
Mitlöhner J, Kaushik R, Niekisch H, Blondiaux A, Gee CE, Happel MFK, Gundelfinger E, Dityatev A, Frischknecht R, Seidenbecher C
Cells (2020) 92: . 173 002 ICC; tested species: rat
Auxiliary α2δ1 and α2δ3 Subunits of Calcium Channels Drive Excitatory and Inhibitory Neuronal Network Development.
Bikbaev A, Ciuraszkiewicz-Wojciech A, Heck J, Klatt O, Freund R, Mitlöhner J, Enrile Lacalle S, Sun M, Repetto D, Frischknecht R, Ablinger C, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4025: 4824-4841. 173 002 ICC; tested species: rat
Biallelic Mutations in TSC2 Lead to Abnormalities Associated with Cortical Tubers in Human iPSC-Derived Neurons.
Winden KD, Sundberg M, Yang C, Wafa SMA, Dwyer S, Chen PF, Buttermore ED, Sahin M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) 3947: 9294-9305. 173 002 ICC; tested species: human
Glia-to-neuron transfer of miRNAs via extracellular vesicles: a new mechanism underlying inflammation-induced synaptic alterations.
Prada I, Gabrielli M, Turola E, Iorio A, D'Arrigo G, Parolisi R, De Luca M, Pacifici M, Bastoni M, Lombardi M, Legname G, et al.
Acta neuropathologica (2018) 1354: 529-550. 173 002 ICC; tested species: mouse
Long-term culture of astrocytes attenuates the readily releasable pool of synaptic vesicles.
Kawano H, Katsurabayashi S, Kakazu Y, Yamashita Y, Kubo N, Kubo M, Okuda H, Takasaki K, Kubota K, Mishima K, Fujiwara M, et al.
PloS one (2012) 710: e48034. 173 002 ICC
Drebrin controls scar formation and astrocyte reactivity upon traumatic brain injury by regulating membrane trafficking.
Schiweck J, Murk K, Ledderose J, Münster-Wandowski A, Ornaghi M, Vida I, Eickholt BJ
Nature communications (2021) 121: 1490. 173 002 ICC, IHC; tested species: mouse
Loss of all three APP family members during development impairs synaptic function and plasticity, disrupts learning, and causes an autism-like phenotype.
Steubler V, Erdinger S, Back MK, Ludewig S, Fässler D, Richter M, Han K, Slomianka L, Amrein I, von Engelhardt J, Wolfer DP, et al.
The EMBO journal (2021) : e107471. 173 002 IHC; tested species: mouse
Glial activation is moderated by sex in response to amyloidosis but not to tau pathology in mouse models of neurodegenerative diseases.
Biechele G, Franzmeier N, Blume T, Ewers M, Luque JM, Eckenweber F, Sacher C, Beyer L, Ruch-Rubinstein F, Lindner S, Gildehaus FJ, et al.
Journal of neuroinflammation (2020) 171: 374. 173 002 IHC; tested species: mouse
Distinct in vivo roles of secreted APP ectodomain variants APPsα and APPsβ in regulation of spine density, synaptic plasticity, and cognition.
Richter MC, Ludewig S, Winschel A, Abel T, Bold C, Salzburger LR, Klein S, Han K, Weyer SW, Fritz AK, Laube B, et al.
The EMBO journal (2018) : . 173 002 WB, IHC; tested species: mouse
Wfs1- deficient rats develop primary symptoms of Wolfram syndrome: insulin-dependent diabetes, optic nerve atrophy and medullary degeneration.
Plaas M, Seppa K, Reimets R, Jagomäe T, Toots M, Koppel T, Vallisoo T, Nigul M, Heinla I, Meier R, Kaasik A, et al.
Scientific reports (2017) 71: 10220. 173 002 IHC; tested species: rat
An electrically resistive sheet of glial cells for amplifying signals of neuronal extracellular recordings.
Matsumura R, Yamamoto H, Niwano M, Hirano-Iwata A
Applied physics letters (2016) 1082: 023701. 173 002 IHC
Large-scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies.
Esposito L, Drexler JF, Braganza O, Doberentz E, Grote A, Widman G, Drosten C, Eis-Hübinger AM, Schoch S, Elger CE, Becker AJ, et al.
Epilepsia (2015) 562: 234-43. 173 002 IHC; tested species: human
βCTF-correlated burst of hippocampal TNFα occurs at a very early, pre-plaque stage in the TgCRND8 mouse model of Alzheimer's disease.
Cavanagh C, Colby-Milley J, Bouvier D, Farso M, Chabot JG, Quirion R, Krantic S
Journal of Alzheimer's disease : JAD (2013) 362: 233-8. 173 002 IHC
Chronic psychosocial stress and citalopram modulate the expression of the glial proteins GFAP and NDRG2 in the hippocampus.
Araya-Callís C, Hiemke C, Abumaria N, Flugge G
Psychopharmacology (2012) 2241: 209-22. 173 002 IHC
Age-dependent kinetics of dentate gyrus neurogenesis in the absence of cyclin D2.
Ansorg A, Witte OW, Urbach A
BMC neuroscience (2012) 13: 46. 173 002 IHC
No improvement after chronic ibuprofen treatment in the 5XFAD mouse model of Alzheimer's disease.
Hillmann A, Hahn S, Schilling S, Hoffmann T, Demuth HU, Bulic B, Schneider-Axmann T, Bayer TA, Weggen S, Wirths O
Neurobiology of aging (2012) 334: 833.e39-50. 173 002 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 173 002 IHC
Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys.
Ribic A, Zhang M, Schlumbohm C, Mätz-Rensing K, Uchanska-Ziegler B, Flügge G, Zhang W, Walter L, Fuchs E
Cellular and molecular neurobiology (2010) 306: 827-39. 173 002 IHC
Coping with AIDS: issues of accountability in the dental office.
Cutler LS
Journal - Connecticut State Dental Association (1988) 621: 20-2. 173 002 IHC; tested species: mouse
Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease.
Hüttenrauch M, Baches S, Gerth J, Bayer TA, Weggen S, Wirths O
Journal of Alzheimer's disease : JAD (2015) 444: 1291-302. 173 002 IHC-P; tested species: mouse
Accelerated tau pathology with synaptic and neuronal loss in a novel triple transgenic mouse model of Alzheimer's disease.
Saul A, Sprenger F, Bayer TA, Wirths O
Neurobiology of aging (2013) 3411: 2564-73. 173 002 IHC-P; tested species: mouse
Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis.
Wirths O, Erck C, Martens H, Harmeier A, Geumann C, Jawhar S, Kumar S, Multhaup G, Walter J, Ingelsson M, Degerman-Gunnarsson M, et al.
The Journal of biological chemistry (2010) 28553: 41517-24. 173 002 IHC-P; tested species: mouse
Accumulation of intraneuronal Abeta correlates with ApoE4 genotype.
Christensen DZ, Schneider-Axmann T, Lucassen PJ, Bayer TA, Wirths O
Acta neuropathologica (2010) 1195: 555-66. 173 002 IHC-P; tested species: human
The Anti-amyloid Compound DO1 Decreases Plaque Pathology and Neuroinflammation-Related Expression Changes in 5xFAD Transgenic Mice.
Boeddrich A, Babila JT, Wiglenda T, Diez L, Jacob M, Nietfeld W, Huska MR, Haenig C, Groenke N, Buntru A, Blanc E, et al.
Cell chemical biology (2018) : . 173 002 ELISA; tested species: mouse
Background
Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises celltype-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.