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VGLUT 1 protein - 135-3P

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
control protein
Cat. No.: 135-3P
Amount: 100 µg
Price: $90.00
Cat. No. 135-3P 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in TBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 135 302, 135 303, 135 303C3, 135 303C5, 135 304, 135 307, 135 311, 135 511
Remarks

This control protein consists of the Strep-Tag® fusion protein (aa 456 - 560 of rat VGLUT 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 135-3p.pdf
Cat. No.: 135-3P
Amount: 100 µg
Price: $90.00
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

Protocols