Cat. No.: 135 511
Amount: 100 µg
Price:
$420.00
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| Cat. No. 135 511 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | 317G6 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Recombinant protein corresponding to residues near the carboxy terminus of rat VGLUT 1 (UniProt Id: Q62634) |
| Epitop |
Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634) |
| Reactivity |
Reacts with: rat (Q62634), mouse (Q3TXX4). Other species not tested yet. |
| Specificity | K.O. validated |
| Matching control protein/peptide | 135-3P |
| Remarks |
WB: This antibody yields weaker signals in Western blot experiments than 135 311, 135 011 and the polyclonal antibodies 135 302, 135 303, 135 304 and 135 306. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. |
| Data sheet | 135_511.pdf |
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The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.