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Home > Products > 119 002

SV2 A antibody - 119 002

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Rabbit polyclonal antiserum
Cat. No.: 119 002
Amount: 200 µl
Price: $360.00
Cat. No. 119 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
EM: yes
Immunogen Synthetic peptide corresponding to AA 2 to 17 from human SV2A (UniProt Id: Q7L0J3)
Reactivity Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5), Guinea pig, cow, hamster, chicken, zebrafish.
Other species not tested yet.
Matching control protein/peptide 119-0P
Remarks

WB:  SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_002.pdf

References for SV2 A - 119 002

SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 119 002 WB; tested species: rat
Abnormal Striatal Development Underlies the Early Onset of Behavioral Deficits in Shank3B-/- Mice.
Peixoto RT, Chantranupong L, Hakim R, Levasseur J, Wang W, Merchant T, Gorman K, Budnik B, Sabatini BL
Cell reports (2019) 297: 2016-2027.e4. 119 002 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 119 002 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 119 002 WB; tested species: mouse
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 119 002 WB; tested species: mouse,rat
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 119 002 WB; tested species: mouse
Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E.
Mahrhold S, Strotmeier J, Garcia-Rodriguez C, Lou J, Marks JD, Rummel A, Binz T
The Biochemical journal (2013) 4531: 37-47. 119 002 WB
Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine.
Couesnon A, Molgó J, Connan C, Popoff MR
PLoS pathogens (2012) 83: e1002583. 119 002 WB
Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam.
Boido D, Farisello P, Cesca F, Ferrea E, Valtorta F, Benfenati F, Baldelli P
Neuroscience (2010) 1711: 268-83. 119 002 WB
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 002 WB; tested species: mouse
Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers.
Couesnon A, Pereira Y, Popoff MR
Cellular microbiology (2008) 102: 375-87. 119 002 WB
SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 002 WB, IHC
The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 119 002 ICC
Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis.
Fossati G, Morini R, Corradini I, Antonucci F, Trepte P, Edry E, Sharma V, Papale A, Pozzi D, Defilippi P, Meier JC, et al.
Cell death and differentiation (2015) 229: 1425-36. 119 002 ICC
Synaptic vesicle glycoprotein 2A modulates vesicular release and calcium channel function at peripheral sympathetic synapses.
Vogl C, Tanifuji S, Danis B, Daniels V, Foerch P, Wolff C, Whalley BJ, Mochida S, Stephens GJ
The European journal of neuroscience (2015) 414: 398-409. 119 002 ICC; KD verified; tested species: rat
A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion and in vitro cell culturing.
Tonna N, Bianco F, Matteoli M, Cagnoli C, Antonucci F, Manfredi A, Mauro N, Ranucci E, Ferruti P
Science and technology of advanced materials (2014) 154: 045007. 119 002 ICC
Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses.
Antonucci F, Corradini I, Morini R, Fossati G, Menna E, Pozzi D, Pacioni S, Verderio C, Bacci A, Matteoli M
EMBO reports (2013) 147: 645-51. 119 002 ICC
Metabolic turnover of synaptic proteins: kinetics, interdependencies and implications for synaptic maintenance.
Cohen LD, Zuchman R, Sorokina O, Müller A, Dieterich DC, Armstrong JD, Ziv T, Ziv NE
PloS one (2013) 85: e63191. 119 002 ICC; tested species: rat
Traffic of botulinum toxins A and E in excitatory and inhibitory neurons.
Verderio C, Grumelli C, Raiteri L, Coco S, Paluzzi S, Caccin P, Rossetto O, Bonanno G, Montecucco C, Matteoli M
Traffic (Copenhagen, Denmark) (2007) 82: 142-53. 119 002 ICC; tested species: rat
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.
Hohsfield LA, Najafi AR, Ghorbanian Y, Soni N, Hingco EE, Kim SJ, Jue AD, Swarup V, Inlay MA, Green KN
Journal of neuroinflammation (2020) 171: 279. 119 002 IHC; tested species: mouse
Changes in neuronal excitability and synaptic transmission in nucleus accumbens in a transgenic Alzheimer's disease mouse model.
Fernández-Pérez EJ, Gallegos S, Armijo-Weingart L, Araya A, Riffo-Lepe NO, Cayuman F, Aguayo LG
Scientific reports (2020) 101: 19606. 119 002 IHC; tested species: mouse
SV2B is essential for the integrity of the glomerular filtration barrier.
Fukusumi Y, Wakamatsu A, Takashima N, Hasegawa E, Miyauchi N, Tomita M, Kawachi H
Laboratory investigation; a journal of technical methods and pathology (2015) 955: 534-45. 119 002 IHC
Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 119 002 IHC; tested species: human
SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 119 002 IHC; tested species: mouse
Immunocytochemical evidence for SNARE protein-dependent transmitter release from guinea pig horizontal cells.
Lee H, Brecha NC
The European journal of neuroscience (2010) 318: 1388-401. 119 002 IHC
A novel cyclic nucleotide-gated ion channel enriched in synaptic terminals of isotocin neurons in zebrafish brain and pituitary.
Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, Matthews G
Neuroscience (2010) 1651: 79-89. 119 002 IHC; tested species: zebrafish
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-beta peptide in mouse brain.
Iwata N, Mizukami H, Shirotani K, Takaki Y, Muramatsu S, Lu B, Gerard NP, Gerard C, Ozawa K, Saido TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2004) 244: 991-8. 119 002 IHC
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 119 002 IHC
SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 002 WB, IHC
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Cat. No.: 119 002
Amount: 200 µl
Price: $360.00
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 119 002 WB; tested species: rat
Abnormal Striatal Development Underlies the Early Onset of Behavioral Deficits in Shank3B-/- Mice.
Peixoto RT, Chantranupong L, Hakim R, Levasseur J, Wang W, Merchant T, Gorman K, Budnik B, Sabatini BL
Cell reports (2019) 297: 2016-2027.e4. 119 002 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 119 002 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 119 002 WB; tested species: mouse
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 119 002 WB; tested species: mouse,rat
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 119 002 WB; tested species: mouse
Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E.
Mahrhold S, Strotmeier J, Garcia-Rodriguez C, Lou J, Marks JD, Rummel A, Binz T
The Biochemical journal (2013) 4531: 37-47. 119 002 WB
Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine.
Couesnon A, Molgó J, Connan C, Popoff MR
PLoS pathogens (2012) 83: e1002583. 119 002 WB
Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam.
Boido D, Farisello P, Cesca F, Ferrea E, Valtorta F, Benfenati F, Baldelli P
Neuroscience (2010) 1711: 268-83. 119 002 WB
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 002 WB; tested species: mouse
Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers.
Couesnon A, Pereira Y, Popoff MR
Cellular microbiology (2008) 102: 375-87. 119 002 WB
SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 002 WB, IHC
The effects of proteasomal inhibition on synaptic proteostasis.
Hakim V, Cohen LD, Zuchman R, Ziv T, Ziv NE
The EMBO journal (2016) 3520: 2238-2262. 119 002 ICC
Reduced SNAP-25 increases PSD-95 mobility and impairs spine morphogenesis.
Fossati G, Morini R, Corradini I, Antonucci F, Trepte P, Edry E, Sharma V, Papale A, Pozzi D, Defilippi P, Meier JC, et al.
Cell death and differentiation (2015) 229: 1425-36. 119 002 ICC
Synaptic vesicle glycoprotein 2A modulates vesicular release and calcium channel function at peripheral sympathetic synapses.
Vogl C, Tanifuji S, Danis B, Daniels V, Foerch P, Wolff C, Whalley BJ, Mochida S, Stephens GJ
The European journal of neuroscience (2015) 414: 398-409. 119 002 ICC; KD verified; tested species: rat
A soluble biocompatible guanidine-containing polyamidoamine as promoter of primary brain cell adhesion and in vitro cell culturing.
Tonna N, Bianco F, Matteoli M, Cagnoli C, Antonucci F, Manfredi A, Mauro N, Ranucci E, Ferruti P
Science and technology of advanced materials (2014) 154: 045007. 119 002 ICC
Reduced SNAP-25 alters short-term plasticity at developing glutamatergic synapses.
Antonucci F, Corradini I, Morini R, Fossati G, Menna E, Pozzi D, Pacioni S, Verderio C, Bacci A, Matteoli M
EMBO reports (2013) 147: 645-51. 119 002 ICC
Metabolic turnover of synaptic proteins: kinetics, interdependencies and implications for synaptic maintenance.
Cohen LD, Zuchman R, Sorokina O, Müller A, Dieterich DC, Armstrong JD, Ziv T, Ziv NE
PloS one (2013) 85: e63191. 119 002 ICC; tested species: rat
Traffic of botulinum toxins A and E in excitatory and inhibitory neurons.
Verderio C, Grumelli C, Raiteri L, Coco S, Paluzzi S, Caccin P, Rossetto O, Bonanno G, Montecucco C, Matteoli M
Traffic (Copenhagen, Denmark) (2007) 82: 142-53. 119 002 ICC; tested species: rat
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS.
Hohsfield LA, Najafi AR, Ghorbanian Y, Soni N, Hingco EE, Kim SJ, Jue AD, Swarup V, Inlay MA, Green KN
Journal of neuroinflammation (2020) 171: 279. 119 002 IHC; tested species: mouse
Changes in neuronal excitability and synaptic transmission in nucleus accumbens in a transgenic Alzheimer's disease mouse model.
Fernández-Pérez EJ, Gallegos S, Armijo-Weingart L, Araya A, Riffo-Lepe NO, Cayuman F, Aguayo LG
Scientific reports (2020) 101: 19606. 119 002 IHC; tested species: mouse
SV2B is essential for the integrity of the glomerular filtration barrier.
Fukusumi Y, Wakamatsu A, Takashima N, Hasegawa E, Miyauchi N, Tomita M, Kawachi H
Laboratory investigation; a journal of technical methods and pathology (2015) 955: 534-45. 119 002 IHC
Expression pattern of synaptic vesicle protein 2 (SV2) isoforms in patients with temporal lobe epilepsy and hippocampal sclerosis.
Crèvecoeur J, Kaminski RM, Rogister B, Foerch P, Vandenplas C, Neveux M, Mazzuferi M, Kroonen J, Poulet C, Martin D, Sadzot B, et al.
Neuropathology and applied neurobiology (2014) 402: 191-204. 119 002 IHC; tested species: human
SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 119 002 IHC; tested species: mouse
Immunocytochemical evidence for SNARE protein-dependent transmitter release from guinea pig horizontal cells.
Lee H, Brecha NC
The European journal of neuroscience (2010) 318: 1388-401. 119 002 IHC
A novel cyclic nucleotide-gated ion channel enriched in synaptic terminals of isotocin neurons in zebrafish brain and pituitary.
Khan S, Perry C, Tetreault ML, Henry D, Trimmer JS, Zimmerman AL, Matthews G
Neuroscience (2010) 1651: 79-89. 119 002 IHC; tested species: zebrafish
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-beta peptide in mouse brain.
Iwata N, Mizukami H, Shirotani K, Takaki Y, Muramatsu S, Lu B, Gerard NP, Gerard C, Ozawa K, Saido TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2004) 244: 991-8. 119 002 IHC
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 119 002 IHC
SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 002 WB, IHC
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
WB
ICC
IHC
IHC-P
Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
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