SNAP 25 - 111 111BT
Palmitoylated plasma membrane protein; required for exocytosis
Monoclonal mouse purified IgG
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|Cat. No. 111 111BT||50 µg purified IgG, lyophilized, biotin-labeled. . For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 10000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 500
IHC: 1 : 200
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)|
Epitop: AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1)
Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals.
No signal: zebrafish.
Other species not tested yet.
|Specificity||Specific for SNAP 25.|
IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 111 002 as detector antibody (protocol for sandwich-ELISA).