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SY SY - Synaptic Systems SY SY - Synaptic Systems

Synaptobrevin 2 (VAMP 2)

Major vesicle protein involved in fusion

Fact Sheet Cat. No. 104 202

Polyclonal rabbit antibody against synaptobrevin2/VAMP 2
Cat. No. 104 202
crude antiserum
200 l antiserum, lyophilized. For reconstitution add 200 l H2O, then aliquot and store at -20C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) blot
IP: yes
ICC: 1 : 500 image
IHC: 1 : 500 image
IHC-P: 1 : 200 image
EM: yes
Immunogen Synthetic peptide SATAATVPPAAPAGEG (aa 2 - 17 in rat synaptobrevin 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Reactivity Human, rat, mouse, hamster; no signal in chicken and zebrafish.
Specificity Specific for VAMP 2, no cross reactivity to VAMP 1 and VAMP 3.
Remarks
  • This antibody recognizes the Botulinumtoxin B cleavage product (aa 1 - 76) with reduced affinity. The sensitivity is sufficient for the detection of cleaved recombinant protein.
  • For analysis of toxin treated tissue homogenates cat. no. 104 203 is recommended.





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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com