mCLING labeling kit - 710-MCK
A fixable membrane probe for high resolution microscopy
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|Cat. No. 710-MCK||
710-MCK A: unlabeled mCling, 50 nmol (lyophilized)
710-MCK B: mCLING purification column
710-MCK C: Washing buffer, 10 ml
710-MCK D: Stop solution, 0.5 ml
710-MCK E: Elution buffer, 1 ml
710-MCK F: Neutralization buffer, 0.5 ml
All components of the kit are stable at 2-8°C.
Shelf life before use: 6 months.
WB: (see remarks)
WB: Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4 %paraformaldehyde (PFA) and 0.2 % glutaraldehyde is strongly advised. For detailed protocols see Revelo NH & Rizzoli SO, 2016.