|Cat. No. 462 004||100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.|
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 250 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: 1 : 500 up to 1 : 100 (see remarks) gallery
|Immunogen||human heterotrimeric Collagen type IV containing two α 1-like (UniProt Id: P02462) and one α 2-like (UniProt Id: P08572) chains.|
Reacts with: mouse (P02463), rat (F1MA59), human (P02462), human (P08572), mouse (P08122), rat (F1M6Q3).
Other species not tested yet.
|Specificity||Specific for Collagen type IV|
IHC: antigen retrieval (0.2 mg/ml pepsin in 0.2 M HCl for 20 min at 37 °C) is recommended.
Type IV collagen is a unique member of the large collagen superfamily which in vertebrates comprise 28 different types. Unlike most collagens, type IV collagen occurs only in the basement membranes (BMs) and comprises up to six genetically distinct α-chains designated α1(IV) to α6(IV). Out of many potential combinations, the chains interact and assemble with a remarkable specificity to form only three distinct heterotrimers of α1α1α2, α3α4α5, and α5α5α6 (1). It has been shown that ablation of COL4 α 1/2 results in abnormal BM structure and embryonic lethality at E10.5–E11.5, although BM formation during early development is unaffected (2).