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EAAT1 antibody extracellular domain - 250 103 K.O.

EAATs are transmembrane proteins involved in the removal of extracellular glutamate
Rabbit polyclonal purified antibody
Cat. No.: 250 103
Amount: 50 µg
Price: $375.00
Cat. No. 250 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 (AP staining) gallery  
IP: not recommended
ICC: not tested yet
IHC: not recommended
IHC-P: not tested yet
Immunogen Synthetic peptide corresponding to AA 186 to 202 from rat EAAT1 (UniProt Id: P24942)
Reactivity Reacts with: rat (P24942), mouse (P56564).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 250-1P

Suitable for immunopanning of dissociated astrocytes.

Data sheet 250_103.pdf
Cat. No.: 250 103
Amount: 50 µg
Price: $375.00
Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. After the release of glutamate from synaptic vesicles into the synaptic cleft during neurotransmission, excitatory amino acid transporters (EAATs) remove extracellular glutamate to avoid excitotoxic levels.
Five EAATs with differential expression patterns have been described so far: EAAT 1, also referred to as GLAST and SLC1A3, has neuroprotective potential following ischemia and is expressed by reactive astrocytes and activated microglia. EAAT 2 (GLT-1, SLC1A2) is the most abundant and primarily expressed in astrocytes. EAAT 3 / SLC1A1 is expressed in neurons and has also been shown to be involved in the uptake of extracelluar cysteine. EAAT 4 shows weak expression in the forebrain and high levels in Purkinje cells of the cerebellum. EAAT 5 has only been described for humans and is primarily expressed in the retina.