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VAChT antibody - 139 009

VAChT is the acetylcholine transporter in the membrane of synaptic vesicles
Chicken monoclonal recombinant IgY
Cat. No.: 139 009
Amount: 50 µg
Price: $415.00
Cat. No. 139 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 up to 1 : 2000 (AP-staining) (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clone Ch15A11
Subtype IgY (λ light chain)
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: mouse (O35304), rat (Q62666).
Other species not tested yet.

This antibody is a chimeric antibody based on the well known monoclonal rat antibody clone 15A11. The constant regions of the heavy and light chains have been replaced with chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
WB: VAChT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 139_009.pdf
Cat. No.: 139 009
Amount: 50 µg
Price: $415.00
The vesicular acetylcholine transporter VAChT is an integral membrane protein with 12 putative trans-membrane domains. VAChT and choline acetyltransferase (ChAT) are encoded by genes organized in a single gene locus, and coregulation of the two genes has been reported several times.
VAChT translocates acetylcholine from the cytoplasm into synaptic vesicles where it stays until release. After release from the presynaptic nerve terminal acetylcholine is hydrolyzed by acetylcholine esterase. During Alzheimer´s disease acetylcholine is one of the first neurotransmitters to be reduced.