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GFP antibody - 132 003C3

GFP is a widely used green fluorescent protein tag
Rabbit polyclonal purified antibody
Cat. No.: 132 003C3
Amount: 50 µg
Price: $480.00
Cat. No. 132 003C3 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with Oyster  550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established fluorescence detection systems.

Oyster 550: λex 551 nm / λem 570 nm
Oyster 650: λex 651 nm / λem 671 nm

Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.
Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Label Oyster 550
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Data sheet 132_003c3.pdf
Cat. No.: 132 003C3
Amount: 50 µg
Price: $480.00
Background
Green fluorescent protein GFP and its derivates have become universal tools in cell biology. These antibodies allow immunoprecipitation and visualization of GFP fusion proteins on immunoblots and by immunocytochemistry.