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SY SY - Synaptic Systems SY SY - Synaptic Systems

VGAT
cytoplasmic domain - 131 008

GABA transporter in the membrane of synaptic vesicles

Recombinant rabbit purified IgG
415.00 USD
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Cat. No. 131 008 50 µg purified IgG, lyophilized. Albumin was added for stabilization.For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 AP staining (see remarks) gallery  
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 up to 1 : 1000 (see remarks) gallery  
Clone Rb117G4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Epitop Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT (K.O. verified)
matching control protein/peptide 131-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

IHC-P: Antigen retrieval at pH 9.0 (10 mM Tris pH 9.0, 1mM EDTA, 0.05 % Tween20) is recommended.

Datasheet 131_008.pdf


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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com