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|Cat. No. 131 008||50 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 2000 AP staining (see remarks) gallery
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 1000 gallery
IHC-P/FFPE: 1 : 500 up to 1 : 1000 (see remarks) gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 75 to 87 from rat VGAT (UniProt Id: O35458)|
Epitop: AA 75 to 87 from rat VGAT (UniProt Id: O35458)
Reacts with: human (Q9H598), rat (O35458), mouse (O35633).
Other species not tested yet.
|Specificity||Specific for VGAT (K.O. verified)|
|matching control protein/peptide||131-0P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 117G4. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
IHC-P: Antigen retrieval at pH 9.0 (10 mM Tris pH 9.0, 1mM EDTA, 0.05 % Tween20) is recommended.