VGAT - 131 005
GABA transporter in the membrane of synaptic vesicles
Polyclonal Guinea pig purified antibody
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|Cat. No. 131 005||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 gallery
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: 1 : 500 gallery
|Immunogen||Recombinant protein corresponding to AA 2 to 115 from rat VGAT (UniProt Id: O35458)|
Reacts with: rat (O35458), mouse (O35633).
Other species not tested yet.
|Specificity||Specific for VGAT|
|matching control protein/peptide||131-0GP|
VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
IHC: Signal intensity can be enhanced by antigen retrieval at pH6 prior to blocking step.