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VGAT - 131 005

GABA transporter in the membrane of synaptic vesicles

Polyclonal Guinea pig purified antibody
450.00 USD
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Cat. No. 131 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 2 to 115 from rat VGAT (UniProt Id: O35458)
Reactivity Reacts with: rat (O35458), mouse (O35633).
Other species not tested yet.
Specificity Specific for VGAT
matching control protein/peptide 131-0GP

VGAT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

IHC: Signal intensity can be enhanced by antigen retrieval at pH6 prior to blocking step.

Datasheet 131_005.pdf

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References for VGAT - 131 005

Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 131 005 ICC; tested species: rat
Corelease of dopamine and GABA by a retinal dopaminergic neuron.
Hirasawa H, Betensky RA, Raviola E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3238: 13281-91. 131 005 IHC; tested species: mouse
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: | E-Mail: