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RIM1 antibody - 140 013 K.D.

RIMs are Rab3 effector proteins of the cytomatrix at the active zone
Rabbit polyclonal purified antibody
Cat. No.: 140 013
Amount: 50 µg
Price: $375.00
Cat. No. 140 013 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes limited (see remarks)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 207 to 366 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity Reacts with: rat (Q9JIR4), mouse (Q99NE5).
Other species not tested yet.
Specificity K.D. validated PubMed: 29230050
Remarks

WB: Cat. no. 140 023 is recommended for westernblotting.

Data sheet 140_013.pdf

References for RIM1 - 140 013

Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 013 WB, IHC; tested species: mouse
Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.
Limbach C, Laue MM, Wang X, Hu B, Thiede N, Hultqvist G, Kilimann MW
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: E392-401. 140 013 WB
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 140 013 ICC; KD verified; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 140 013 ICC; tested species: rat
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 140 013 IHC; tested species: mouse
Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 013 WB, IHC; tested species: mouse
Cat. No.: 140 013
Amount: 50 µg
Price: $375.00
Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 013 WB, IHC; tested species: mouse
Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.
Limbach C, Laue MM, Wang X, Hu B, Thiede N, Hultqvist G, Kilimann MW
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: E392-401. 140 013 WB
Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 140 013 ICC; KD verified; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 140 013 ICC; tested species: rat
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 140 013 IHC; tested species: mouse
Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 013 WB, IHC; tested species: mouse
Background

RIMs are presynaptic active zone proteins that regulate Ca2+ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.