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SY SY - Synaptic Systems SY SY - Synaptic Systems

Bassoon - 141 011

A large multi-domain protein of the presynaptic active zone

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 141 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC: not recommended
IHC-P/FFPE: 1 : 500 gallery  
Clone 219E1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Epitop Epitop: AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Reactivity Reacts with: rat, mouse.
Other species not tested yet.
Specificity Specific for bassoon.
matching control protein/peptide 141-0P
Remarks

WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended.

Datasheet 141_011.pdf


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References for Bassoon - 141 011

Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
Postsynaptic Y654 dephosphorylation of β-catenin modulates presynaptic vesicle turnover through increased n-cadherin-mediated transsynaptic signaling.
Chen CY, Chen YT, Wang JY, Huang YS, Tai CY
Developmental neurobiology (2017) 771: 61-74. 141 011 WB, IP
eIF4B phosphorylation at Ser504 links synaptic activity with protein translation in physiology and pathology.
Bettegazzi B, Bellani S, Roncon P, Guarnieri FC, Bertero A, Codazzi F, Valtorta F, Simonato M, Grohovaz F, Zacchetti D
Scientific reports (2017) 71: 10563. 141 011 ICC; tested species: rat
Remodelling of spared proprioceptive circuit involving a small number of neurons supports functional recovery.
Hollis ER, Ishiko N, Pessian M, Tolentino K, Lee-Kubli CA, Calcutt NA, Zou Y
Nature communications (2015) 6: 6079. 141 011 IHC
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com