Bassoon - 141 011
A large multi-domain protein of the presynaptic active zone
Monoclonal mouse purified IgG
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|Cat. No. 141 011||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC: not recommended
IHC-P/FFPE: 1 : 500 gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)|
Epitop: AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778)
Reacts with: rat (O88778), mouse (O88737).
Other species not tested yet.
|Specificity||Specific for bassoon.|
|matching control protein/peptide||141-0P|
WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended.