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PSD95 sdAb - FluoTag-X2 - N3702-AF568-L

PSD95 is a component of postsynaptic densities in central synapses

This product was developed by   

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3702-AF568-L
Amount: 200 µl
Price: $515.00
Cat. No. N3702-AF568-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with AZdye 568.

Fluorescence labeled antibodies conjugated to Alexa® dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

AZdye® 568: λex 568 nm / λem 665 nm (identical to Alexa® 568)
Alexa® 647: λex 650 nm / λem 665 nm

This product or portions thereof is manufactured under license from Life Technologies Corporation. 
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation.

Albumin was added for stabilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: yes
ICC: yes gallery  
IHC: yes
Label AZdye 568, two fluorophores coupled to one FluoTag
Clone 1B2
Immunogen Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)
Reactivity Reacts with: mouse (Q62108), rat (P31016), human (P78352).
Other species not tested yet.
Specificity Recognizes mouse and rat PSD95. Other species not tested
Matching control protein/peptide 124-01P

References for PSD95 sdAb - N3702-AF568-L

Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N3702-AF568-L ICC, IHC; tested species: mouse,rat
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N3702-AF568-L ICC, IHC; tested species: mouse,rat

Cat. No.: N3702-AF568-L
Amount: 200 µl
Price: $515.00
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N3702-AF568-L ICC, IHC; tested species: mouse,rat
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives.
Kilisch M, Gere-Becker M, Wüstefeld L, Bonnas C, Crauel A, Mechmershausen M, Martens H, Götzke H, Opazo F, Frey S
International journal of molecular sciences (2023) 248: . N3702-AF568-L ICC, IHC; tested species: mouse,rat
Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.