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Tight junction protein ZO-1 antibody - 474 005

ZO-1 is a scaffold protein involved in tight junction assembly
Guinea pig polyclonal purified antibody
Cat. No.: 474 005
Amount: 50 µg
Price: $460.00
Cat. No. 474 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not recommended
IHC-P: not tested yet
IHC-Fr: 1 : 100 up to 1 : 2500 (see remarks) gallery  
Immunogen Recombinant protein corresponding to residues near the carboxy terminus of mouse Tight junction protein ZO-1. (UniProt Id: P39447)
Reactivity Reacts with: mouse (P39447), rat (A0A0G2K2P5), dog (O97758).
Other species not tested yet.
Matching control protein/peptide 474-0P

IHC-Fr: Methanol fixation is recommended.

Data sheet 474_005.pdf
Cat. No.: 474 005
Amount: 50 µg
Price: $460.00

ZO-1 (also known as TJP1) is a peripheral membrane phosphoprotein with a molecular mass of 220 kDa, located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells. (1) Two isoforms, ZO-1α− and ZO-1α+, have been described, which, as a result of alternative RNA splicing, differ by an internal 80 amino acid sequence. (2) 
ZO-1 binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin. (3)