Synaptic Systems - Aquaporin4

Cat. No.: 429 004

Amount: 100 µl

Price: $375.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Cat. No. 429 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 5000 up to 1 : 10000 (AP staining) gallery IP: yes ICC: 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P (FFPE): 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Aquaporin-4 isoform 2 (UniProt Id: P55088-1)
Reactivity	Reacts with: rat (P47863), mouse (P55088-1). Other species not tested yet.
Specificity	Specific for Aquaporin- 4, detects all described isoforms.
Matching control protein/peptide	429-0P
Data sheet	Data Sheet 429_004 Safety Data Sheet (SDS)

References for Aquaporin4 - 429 004

Adenosine A2A Receptors Control the Mis-Localization of Aquaporin-4 in Rats Subject to Repeated Restraint Stress.
Dias L, Ferreira SG, Nabais AM, Silva J, Cunha RA, Agostinho P
Journal of neurochemistry (2026) 1704: e70450. 429 004 WB, IHC; tested species: rat

Vascular Development of Fetal and Postnatal Neocortex of the Pig, the European Wild Boar Sus scrofa.
Sobierajski E, Czubay K, Beemelmans C, Beemelmans C, Meschkat M, Uhlenkamp D, Meyer G, Wahle P
The Journal of comparative neurology (2024) 53212: e70011. 429 004 WB, IHC; tested species: pig

Evaluation of gliovascular functions of Aqp4 readthrough isoforms.
Mueller SM, White KM, Fass SB, Chen S, Shi Z, Ge X, Engelbach JA, Gaines SH, Bice AR, Vasek MJ, Garbow JR, et al.
bioRxiv : the preprint server for biology (2023) : . 429 004 WB; tested species: mouse

40 Hz light flickering facilitates the glymphatic flow via adenosine signaling in mice.
Sun X, Dias L, Peng C, Zhang Z, Ge H, Wang Z, Jin J, Jia M, Xu T, Guo W, Zheng W, et al.
Cell discovery (2024) 101: 81. 429 004 IP; tested species: mouse

Astrocyte TrkB promotes brain injury and edema formation in ischemic stroke.
Colombo E, Bacigaluppi M, Bartoccetti M, Triolo D, Bassani C, Bergamaschi A, Descamps HC, Gullotta GS, Henley M, Piccoli M, Anastasia L, et al.
Neurobiology of disease (2024) 201: 106670. 429 004 IHC; tested species: mouse

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 429 004 IHC; tested species: human

Microglia directly associate with pericytes in the central nervous system.
Morris GP, Foster CG, Courtney JM, Collins JM, Cashion JM, Brown LS, Howells DW, DeLuca GC, Canty AJ, King AE, Ziebell JM, et al.
Glia (2023) : . 429 004 IHC; tested species: mouse

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 429 004 IHC; tested species: mouse

Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 429 004 IHC; tested species: mouse

Update on Perineuronal Net Staining With Wisteria floribunda Agglutinin (WFA).
Härtig W, Meinicke A, Michalski D, Schob S, Jäger C
Frontiers in integrative neuroscience (2022) 16: 851988. 429 004 IHC; tested species: mouse

SARS-CoV-2 infects neurons and induces neuroinflammation in a non-human primate model of COVID-19.
Beckman D, Bonillas A, Diniz GB, Ott S, Roh JW, Elizaldi SR, Schmidt BA, Sammak RL, Van Rompay KKA, Iyer SS, Morrison JH, et al.
Cell reports (2022) 415: 111573. 429 004 IHC; tested species: monkey

Regionally Altered Immunosignals of Surfactant Protein-G, Vascular and Non-Vascular Elements of the Neurovascular Unit after Experimental Focal Cerebral Ischemia in Mice, Rats, and Sheep.
Michalski D, Reimann W, Spielvogel E, Mages B, Biedermann B, Barthel H, Nitzsche B, Schob S, Härtig W
International journal of molecular sciences (2022) 2311: . 429 004 IHC; tested species: mouse

Microglia dysfunction, neurovascular inflammation and focal neuropathologies are linked to IL-1- and IL-6-related systemic inflammation in COVID-19.
Fekete R, Simats A, Bíró E, Pósfai B, Cserép C, Schwarcz AD, Szabadits E, Környei Z, Tóth K, Fichó E, Szalma J, et al.
Nature neuroscience (2025) 283: 558-576. 429 004 IHC-P (FFPE); tested species: human

Cat. No.: 429 004

Amount: 100 µl

Price: $375.00

Background

Aquaporin 4 (AQP4) is the most abundant bidirectional water-channel in the central nervous system. It is particularly abundant in astrocytes, where it is highly concentrated in the perivascular end-feet surrounding blood vessels and at the interfaces between brain tissue and cerebrospinal fluid (1). AQP4 is also expressed in ependymal cells and, outside the CNS, in tissues including the kidney, skeletal muscle, lung, stomach and several glandular epithelia (2,3).
It contributes to the regulation of water and ion homeostasis, extracellular volume, brain edema and blood–brain barrier function. AQP4 is also an important component of the glymphatic system, which facilitates the movement and clearance of substances through the brain interstitial space (1,4).
Alterations in AQP4 expression or localization have been implicated in a number of pathological conditions including neuroinflammation, brain edema, traumatic brain injury, neurodegenerative disorders and neuromyelitis optica spectrum disorders (NMOSD)(4).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Nagelhus EA et al., Physiol. Rev. (2013)
2 ) Palazzo C et al., Int J Mol Sci (2020)
3 ) Gleiser C et al., Int J Mol Sci (2016)
4 ) Wang Y et al., Aging Dis (2022)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Standard Condition

Other products for this target

Aquaporin4 antibody - 429 004

References for Aquaporin4 - 429 004