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beta-Actin antibody - 251 011C3

beta-Actin is a major constituent of the cytoskeleton
Monoclonal mouse purified IgG
Cat. No.: 251 011C3
Amount: 100 µg
Price: $465.00
Cat. No. 251 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Oyster® 550

Fluorescence labeled antibodies conjugated to Oyster dyes from Luminartis GmbH (formerly Denovo Biolabels) allow the usage of established CyDye fluorescence detection systems. Oyster-488 has similar exitation and emission properties as Cy2, Oyster-550 as Cy3, and Oyster-650 is comparable to Cy5.
For our custom antibody labeling service Oyster-500 is also available.
Oyster dyes do not form dimers and therefore exhibit less internal quenching. This allows a higher labeling degree and consequently brighter conjugates.

Oyster dyes, Oyster-488, Oyster-500, Oyster-550, and Oyster-650 are trademarks of Luminartis GmbH, Muenster/Germany.

CyDyes, Cy2, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.The mounting agent Aquatex®(Merck Chemicals) is not compatible with Oyster dyes!
Applications WB: N/A
IP: N/A
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label Oyster 550
Clone 130B4
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 2 to 16 from mouse β-Actin (UniProt Id: P60710)
Epitop Epitop: AA 2 to 14 from mouse β-Actin (UniProt Id: P60710)
Reactivity Reacts with: rat, mouse (P60710), zebrafish.
Other species not tested yet.
Specificity May cross-react to α- and γ-actin due to sequence homology.
Remarks

ICC: methanol or PFA fixation

Data sheet 251_011c3.pdf
Cat. No.: 251 011C3
Quantity: 100 µg
Price: $465.00
Background
The two major cytoskeletal proteins involved in cell motility are myosin and actin. Monomeric actin is a globular protein that is expressed in all eukaryotic cells. Actin is the major subunit of microfilaments, a major component of the cytoskeleton, and of thin filaments, part of the contractile apparatus in muscle cells.
Actin is involved in many cellular processes including cell motility, maintenance of cell shape, and organelle trafficking.
Three main groups of actin have been identified. α-actins are found in muscle tissues whereas β- and γ-actins co-exist in most cell types as components of the cytoskeleton.