Cat. No.: 173 011BT
Amount: 100 µg
Price:
$470.00
Cat. No. 173 011BT |
100 µg purified IgG, lyophilized, labeled with Biotin.
Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
Applications |
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 200 gallery Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 200 gallery |
Label | biotin |
Clone | 134B1 |
Subtype | IgG2a (κ light chain) |
Immunogen | full-length recombinant human GFAP (UniProt Id: P14136) |
Epitop |
Epitop: AA 391 to 405 from human GFAP (UniProt Id: P14136) |
Reactivity |
Reacts with: human (P14136), rat (P47819), mouse (P03995), cow. No signal: zebrafish. Other species not tested yet. |
Specificity | Specific for GFAP isoform 1 (alpha). K.O. validated |
Matching control protein/peptide | 173-0P |
Remarks |
WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002). |
Data sheet | 173_011bt.pdf |