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Homer 3 antibody - 160 303

Homer1 , 2 and 3 are post synaptic density (PSD) proteins involved in the targeting of glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 160 303
Amount: 50 µg
Price: $370.00
Cat. No. 160 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 250 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Clarity: 1 : 300 (see remarks)
Immunogen Recombinant protein corresponding to AA 1 to 177 from rat Homer3 (UniProt Id: Q9Z2X5)
Reactivity Reacts with: rat (Q9Z2X5).
Other species not tested yet.
Specificity AA 122-177, unique for homer 3, were used for affinity purification.
Matching control protein/peptide 160-3P
Remarks

Clarity: This antibody has been successfully used for CLARITY application in human brain (Woelfle et al. 2022. bioRxiv)

Data sheet 160_303.pdf

References for Homer 3 - 160 303

Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 160 303 WB; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex.
Glas A, Hübener M, Bonhoeffer T, Goltstein PM
PloS one (2019) 144: e0214954. 160 303 IHC; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Cat. No.: 160 303
Amount: 50 µg
Price: $370.00
Adult restoration of Shank3 expression rescues selective autistic-like phenotypes.
Mei Y, Monteiro P, Zhou Y, Kim JA, Gao X, Fu Z, Feng G
Nature (2016) 5307591: 481-4. 160 303 WB; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex.
Glas A, Hübener M, Bonhoeffer T, Goltstein PM
PloS one (2019) 144: e0214954. 160 303 IHC; tested species: mouse
Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation.
Tao-Cheng JH, Thein S, Yang Y, Reese TS, Gallant PE
Neuroscience (2014) 266: 80-90. 160 303 WB, EM
Background

Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, Homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.