|Cat. No. 160 011BT||100 µg purified IgG, lyophilized, biotin-labeled. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining)
ICC: 1 : 100 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)|
Epitop: AA 1 to 168 from human Homer1 (UniProt Id: Q86YM7)
Reacts with: human (Q86YM7), rat (Q9Z214), mouse (Q9Z2Y3).
Other species not tested yet.
|Specificity||Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.|
|Matching control protein/peptide||160-0P|
Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.