|Cat. No. 160 008||50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 196 from human Homer1 (UniProt Id: Q86YM7)|
Epitop: AA 1 to 168 from human Homer1 (UniProt Id: Q86YM7)
Reacts with: rat (Q9Z214), mouse (Q9Z2Y3), human (Q86YM7).
Other species not tested yet.
|Specificity||Specific for Homer 1. According to Soloviev et al. (2000), aa 1 - 180 are present in isoforms a, b, c and d.|
|Matching control protein/peptide||160-0P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 2G8. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Homer is a scaffolding protein of the post synaptic density (PSD) and enriched at excitatory synapses. The protein binds metabotropic glutamate receptors, TRPC1, proteins of the Shank family and others. By aggregating these proteins into clusters, homer was suggested to organize distinct signalling domains.
Three isoforms, Homer 1, 2 and 3 have been described. Each of these isoforms is subject to alternative splicing yielding the splice variants a, b, c, d.