Synaptic Systems

Cat. No.: 140 013

Amount: 50 µg

Price: $375.00

Cat. No. 140 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: yes limited (see remarks) IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 207 to 366 from rat Rim1 (UniProt Id: Q9JIR4)
Reactivity	Reacts with: rat (Q9JIR4), mouse (Q99NE5). Other species not tested yet.
Specificity	K.D. validated PubMed: 29230050
Remarks	WB: Cat. no. 140 023 is recommended for westernblotting.
Data sheet	140_013.pdf

References for RIM1 - 140 013

Analysis of RIM Expression and Function at Mouse Photoreceptor Ribbon Synapses.
Löhner M, Babai N, Müller T, Gierke K, Atorf J, Joachimsthaler A, Peukert A, Martens H, Feigenspan A, Kremers J, Schoch S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3733: 7848-7863. 140 013 WB, IHC; tested species: mouse

Molecular in situ topology of Aczonin/Piccolo and associated proteins at the mammalian neurotransmitter release site.
Limbach C, Laue MM, Wang X, Hu B, Thiede N, Hultqvist G, Kilimann MW
Proceedings of the National Academy of Sciences of the United States of America (2011) 10831: E392-401. 140 013 WB

cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
Wang XT, Zhou L, Dong BB, Xu FX, Wang DJ, Shen EW, Cai XY, Wang Y, Wang N, Ji SJ, Chen W, et al.
eLife (2023) 12: . 140 013 IP, ICC; tested species: mouse

Synaptic weight set by Munc13-1 supramolecular assemblies.
Sakamoto H, Ariyoshi T, Kimpara N, Sugao K, Taiko I, Takikawa K, Asanuma D, Namiki S, Hirose K
Nature neuroscience (2018) 211: 41-49. 140 013 ICC; KD verified; tested species: rat

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 140 013 ICC; tested species: rat

Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 140 013 IHC; tested species: mouse

Cat. No.: 140 013

Amount: 50 µg

Price: $375.00

Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 140 013 ICC; tested species: rat

ICC

IHC

Background

RIMs are presynaptic active zone proteins that regulate Ca²⁺ triggered release of neurotransmitters. RIM 1α and RIM 2α are composed of an N-terminal zinc-finger domain, a central PDZ domain and two C-terminal C2 domains that are seperated by long alternatively spliced sequences.
RIM 1α is a putative Rab 3a effector and has been shown to interact with other active zone proteins like Munc13-1, ERC 1b, ERC 2 and α-liprins. Deletion of RIM 1α in mice impaired neurotransmitter release without changing the structure of the synapse.

1 ) Wang Y et al., Genomics (2003)
2 ) Castillo PE et al., Nature (2002)
3 ) Schoch S et al., Nature (2002)
4 ) Wang Y et al., J. Biol. Chem. (2000)
5 ) Wang Y et al., Nature (1997)

Protocols

ICC

IHC

Other products for this target

RIM1 antibody - 140 013 K.D.

References for RIM1 - 140 013