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VGLUT 1 antibody - 135 011

VGluT1 is a glutamate transporter in the membrane of synaptic vesicles
Mouse monoclonal purified IgG
Cat. No.: 135 011
Amount: 100 µg
Price: $415.00
Cat. No. 135 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000      gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone 68B7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat VGLUT 1. (UniProt Id: Q62634)
Epitop Epitop: AA 542 to 560 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1 K.O.
Matching control protein/peptide 135-0P
Remarks

WB: This antibody is highly recommended for Western blot experiments. VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 135_011.pdf

References for VGLUT 1 - 135 011

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 011 WB, app.uptake; tested species: rat
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 135 011 WB; tested species: rat
CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation.
Dong Z, Chen W, Chen C, Wang H, Cui W, Tan Z, Robinson H, Gao N, Luo B, Zhang L, Zhao K, et al.
Neuron (2020) 1053: 475-490.e6. 135 011 WB; tested species: mouse
Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 135 011 WB; tested species: mouse
Long-Term Cultures of Spinal Cord Interneurons.
Vargova I, Kriska J, Kwok JCF, Fawcett JW, Jendelova P
Frontiers in cellular neuroscience (2022) 16: 827628. 135 011 ICC; tested species: mouse
Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids.
Trujillo CA, Adams JW, Negraes PD, Carromeu C, Tejwani L, Acab A, Tsuda B, Thomas CA, Sodhi N, Fichter KM, Romero S, et al.
EMBO molecular medicine (2021) 131: e12523. 135 011 ICC; tested species: human
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 135 011 ICC; tested species: mouse
Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior.
Klatt O, Repetto D, Brockhaus J, Reissner C, El Khallouqi A, Rohlmann A, Heine M, Missler M
Cell reports (2021) 3511: 109266. 135 011 ICC; tested species: mouse
Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency.
Frew J, Baradaran-Heravi A, Balgi AD, Wu X, Yan TD, Arns S, Shidmoossavee FS, Tan J, Jaquith JB, Jansen-West KR, Lynn FC, et al.
Molecular neurodegeneration (2020) 151: 21. 135 011 ICC; tested species: human
Altered fast synaptic transmission in a mouse model of DNM1-associated developmental epileptic encephalopathy.
McCabe MP, Shore AN, Frankel WN, Weston MC
eNeuro (2020) : . 135 011 ICC; tested species: mouse
Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological.
Upadhyay A, Hosseinibarkooie S, Schneider S, Kaczmarek A, Torres-Benito L, Mendoza-Ferreira N, Overhoff M, Rombo R, Grysko V, Kye MJ, Kononenko NL, et al.
Frontiers in molecular neuroscience (2019) 12: 19. 135 011 ICC; tested species: mouse
Setd5 haploinsufficiency alters neuronal network connectivity and leads to autistic-like behaviors in mice.
Moore SM, Seidman JS, Ellegood J, Gao R, Savchenko A, Troutman TD, Abe Y, Stender J, Lee D, Wang S, Voytek B, et al.
Translational psychiatry (2019) 91: 24. 135 011 ICC; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 011 WB, app.uptake; tested species: rat
Cat. No.: 135 011
Amount: 100 µg
Price: $415.00
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 011 WB, app.uptake; tested species: rat
Distinct insulin granule subpopulations implicated in the secretory pathology of diabetes types 1 and 2.
Kreutzberger AJB, Kiessling V, Doyle CA, Schenk N, Upchurch CM, Elmer-Dixon M, Ward AE, Preobraschenski J, Hussein SS, Tomaka W, Seelheim P, et al.
eLife (2020) 9: . 135 011 WB; tested species: rat
CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation.
Dong Z, Chen W, Chen C, Wang H, Cui W, Tan Z, Robinson H, Gao N, Luo B, Zhang L, Zhao K, et al.
Neuron (2020) 1053: 475-490.e6. 135 011 WB; tested species: mouse
Synaptic zinc contributes to motor and cognitive deficits in 6-hydroxydopamine mouse models of Parkinson's disease.
Sikora J, Kieffer BL, Paoletti P, Ouagazzal AM
Neurobiology of disease (2019) 134: 104681. 135 011 WB; tested species: mouse
Long-Term Cultures of Spinal Cord Interneurons.
Vargova I, Kriska J, Kwok JCF, Fawcett JW, Jendelova P
Frontiers in cellular neuroscience (2022) 16: 827628. 135 011 ICC; tested species: mouse
Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids.
Trujillo CA, Adams JW, Negraes PD, Carromeu C, Tejwani L, Acab A, Tsuda B, Thomas CA, Sodhi N, Fichter KM, Romero S, et al.
EMBO molecular medicine (2021) 131: e12523. 135 011 ICC; tested species: human
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 135 011 ICC; tested species: mouse
Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior.
Klatt O, Repetto D, Brockhaus J, Reissner C, El Khallouqi A, Rohlmann A, Heine M, Missler M
Cell reports (2021) 3511: 109266. 135 011 ICC; tested species: mouse
Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency.
Frew J, Baradaran-Heravi A, Balgi AD, Wu X, Yan TD, Arns S, Shidmoossavee FS, Tan J, Jaquith JB, Jansen-West KR, Lynn FC, et al.
Molecular neurodegeneration (2020) 151: 21. 135 011 ICC; tested species: human
Altered fast synaptic transmission in a mouse model of DNM1-associated developmental epileptic encephalopathy.
McCabe MP, Shore AN, Frankel WN, Weston MC
eNeuro (2020) : . 135 011 ICC; tested species: mouse
Neurocalcin Delta Knockout Impairs Adult Neurogenesis Whereas Half Reduction Is Not Pathological.
Upadhyay A, Hosseinibarkooie S, Schneider S, Kaczmarek A, Torres-Benito L, Mendoza-Ferreira N, Overhoff M, Rombo R, Grysko V, Kye MJ, Kononenko NL, et al.
Frontiers in molecular neuroscience (2019) 12: 19. 135 011 ICC; tested species: mouse
Setd5 haploinsufficiency alters neuronal network connectivity and leads to autistic-like behaviors in mice.
Moore SM, Seidman JS, Ellegood J, Gao R, Savchenko A, Troutman TD, Abe Y, Stender J, Lee D, Wang S, Voytek B, et al.
Translational psychiatry (2019) 91: 24. 135 011 ICC; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 135 011 WB, app.uptake; tested species: rat
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.