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PSD95 antibody PDZ domain - 124 008

PSD 95 is a component of postsynaptic densities in central synapses
Rabbit monoclonal recombinant IgG
Cat. No.: 124 008
Amount: 50 µg
Price: $415.00
Cat. No. 124 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Clone Rb108E10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 68 to 251 from mouse PSD95 (UniProt Id: Q62108)
Reactivity Reacts with: rat (P31016), mouse (Q62108), chicken.
Other species not tested yet.
Specificity Specific for PSD 95. K.O.
Matching control protein/peptide 124-01P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 108E10. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IP: For most effective IP use the denaturing SDS based solubilization protocol. Consider that protein-protein interactions may be affected.

Data sheet 124_008.pdf

References for PSD95 - 124 008

Roles of glutamate receptors in a novel in vitro model of early, comorbid cerebrovascular, and Alzheimer's diseases.
Simões-Pires EN, Ferreira ST, Linden R
Journal of neurochemistry (2020) : . 124 008 WB, IHC; tested species: rat
Roles of glutamate receptors in a novel in vitro model of early, comorbid cerebrovascular, and Alzheimer's diseases.
Simões-Pires EN, Ferreira ST, Linden R
Journal of neurochemistry (2020) : . 124 008 WB, IHC; tested species: rat
Cat. No.: 124 008
Amount: 50 µg
Price: $415.00
Roles of glutamate receptors in a novel in vitro model of early, comorbid cerebrovascular, and Alzheimer's diseases.
Simões-Pires EN, Ferreira ST, Linden R
Journal of neurochemistry (2020) : . 124 008 WB, IHC; tested species: rat
Roles of glutamate receptors in a novel in vitro model of early, comorbid cerebrovascular, and Alzheimer's diseases.
Simões-Pires EN, Ferreira ST, Linden R
Journal of neurochemistry (2020) : . 124 008 WB, IHC; tested species: rat
Background

PSD95 (postsynaptic density protein 95 kDa, also called SAP 90: synapse associated protein of 90 kDa and DLG 4) is a component of postsynaptic densities in central synapses. It contains three PDZ domains. The first and second PDZ domain localizes NMDA receptors and K+ channels to synapses, the third binds to neuroligins which are neuronal cell adhesion molecules that interact with β-neurexins and form intercellular junctions. Thus different PDZ domains of PSD 95 might be specialized for distinct functions. Read more: PSD95 single domain antibody