Synaptic Systems - Amphiphysin

Cat. No.: 120 003

Amount: 50 µg

Price: $460.00

Cat. No. 120 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)
Reactivity	Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken. Other species not tested yet.
Specificity	K.D. validated PubMed: 37243578
Matching control protein/peptide	120-0P
Data sheet	Datasheet 120_003

References for Amphiphysin - 120 003

The phospho-regulated amphiphysin/endophilin interaction is required for synaptic vesicle endocytosis.
Kontaxi C, Kim N, Cousin MA
Journal of neurochemistry (2023) 1662: 248-264. 120 003 ICC; KD verified; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 120 003 DNA-PAINT; tested species: rat

Cat. No.: 120 003

Amount: 50 µg

Price: $460.00

ICC

Background

Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.

1 ) Slepnev VI et al., Nat. Rev. Neurosci. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Marsh M et al., Science (1999)
4 ) Wigge P et al., Trends Neurosci. (1998)
5 ) Wigge P et al., Mol. Biol. Cell (1997)
6 ) David C et al., Proc. Natl. Acad. Sci. U.S.A. (1996)
7 ) Südhof TC et al., Nature (1995)
8 ) De Camilli P et al., J. Exp. Med. (1993)
9 ) Lichte B et al., EMBO J. (1992)

Protocols

ICC

Fixed Cells

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Amphiphysin antibody - 120 003 K.D.

References for Amphiphysin - 120 003