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Syntaxin 4 antibody - 110 042

Syntaxin4 is an ubiquitous plasma membrane protein involved in fusion of vesicles with the plasma membrane
Polyclonal rabbit antiserum
Cat. No.: 110 042
Amount: 200 µl
Price: $350.00
Cat. No. 110 042 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not recommended
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 273 from rat Syntaxin4 (UniProt Id: Q08850)
Reactivity Reacts with: human (Q12846), rat (Q08850), mouse (P70452), hamster.
Other species not tested yet.
Specificity Specific for syntaxin 4.
Matching control protein/peptide 110-4P
Data sheet 110_042.pdf

References for Syntaxin 4 - 110 042

Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 110 042 WB, IP; tested species: human
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 042 WB; tested species: human
Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 110 042 WB, ICC; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 042 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 110 042 WB; tested species: mouse
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, et al.
The Journal of biological chemistry (2018) 2937: 2510-2522. 110 042 WB, IP; tested species: mouse
Limited proteolysis as a tool to probe the tertiary conformation of dysferlin and structural consequences of patient missense variant L344P.
Woolger N, Bournazos A, Sophocleous RA, Evesson FJ, Lek A, Driemer B, Sutton RB, Cooper ST
The Journal of biological chemistry (2017) 29245: 18577-18591. 110 042 WB; tested species: human
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 110 042 WB, ICC
Tumor protein D52 controls trafficking of an apical endolysosomal secretory pathway in pancreatic acinar cells.
Messenger SW, Thomas DD, Falkowski MA, Byrne JA, Gorelick FS, Groblewski GE
American journal of physiology. Gastrointestinal and liver physiology (2013) 3056: G439-52. 110 042 WB; tested species: rat
Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.
Arasaki K, Roy CR
Traffic (Copenhagen, Denmark) (2010) 115: 587-600. 110 042 WB, ICC; tested species: human
Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini.
Falkowski MA, Thomas DD, Groblewski GE
The Journal of biological chemistry (2010) 28546: 35558-66. 110 042 WB
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 110 042 WB, ICC; tested species: rat
Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes.
Liu L, Jedrychowski MP, Gygi SP, Pilch PF
Molecular biology of the cell (2006) 1710: 4249-56. 110 042 WB, ICC; tested species: rat
Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 110 042 WB, IP; tested species: human
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, et al.
The Journal of biological chemistry (2018) 2937: 2510-2522. 110 042 WB, IP; tested species: mouse
Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 110 042 WB, ICC; tested species: mouse
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 110 042 WB, ICC
Calpains, cleaved mini-dysferlinC72, and L-type channels underpin calcium-dependent muscle membrane repair.
Lek A, Evesson FJ, Lemckert FA, Redpath GM, Lueders AK, Turnbull L, Whitchurch CB, North KN, Cooper ST
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3312: 5085-94. 110 042 ICC; tested species: human
Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.
Arasaki K, Roy CR
Traffic (Copenhagen, Denmark) (2010) 115: 587-600. 110 042 WB, ICC; tested species: human
Reduced plasma membrane expression of dysferlin mutants is attributed to accelerated endocytosis via a syntaxin-4-associated pathway.
Evesson FJ, Peat RA, Lek A, Brilot F, Lo HP, Dale RC, Parton RG, North KN, Cooper ST
The Journal of biological chemistry (2010) 28537: 28529-39. 110 042 ICC; tested species: human
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 110 042 WB, ICC; tested species: rat
Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes.
Liu L, Jedrychowski MP, Gygi SP, Pilch PF
Molecular biology of the cell (2006) 1710: 4249-56. 110 042 WB, ICC; tested species: rat
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 110 042
Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis.
Li G, Alexander EA, Schwartz JH
The Journal of biological chemistry (2003) 27822: 19791-7. 110 042
From lysosomes to the plasma membrane: localization of vacuolar-type H+ -ATPase with the a3 isoform during osteoclast differentiation.
Toyomura T, Murata Y, Yamamoto A, Oka T, Sun-Wada GH, Wada Y, Futai M
The Journal of biological chemistry (2003) 27824: 22023-30. 110 042
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 042
Cat. No.: 110 042
Quantity: 200 µl
Price: $350.00
Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 110 042 WB, IP; tested species: human
SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors.
Koike S, Jahn R
Nature communications (2019) 101: 1608. 110 042 WB; tested species: human
Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 110 042 WB, ICC; tested species: mouse
Cardiac SNARE Expression in Health and Disease.
Bowman PRT, Smith GL, Gould GW
Frontiers in endocrinology (2019) 10: 881. 110 042 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 110 042 WB; tested species: mouse
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, et al.
The Journal of biological chemistry (2018) 2937: 2510-2522. 110 042 WB, IP; tested species: mouse
Limited proteolysis as a tool to probe the tertiary conformation of dysferlin and structural consequences of patient missense variant L344P.
Woolger N, Bournazos A, Sophocleous RA, Evesson FJ, Lek A, Driemer B, Sutton RB, Cooper ST
The Journal of biological chemistry (2017) 29245: 18577-18591. 110 042 WB; tested species: human
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 110 042 WB, ICC
Tumor protein D52 controls trafficking of an apical endolysosomal secretory pathway in pancreatic acinar cells.
Messenger SW, Thomas DD, Falkowski MA, Byrne JA, Gorelick FS, Groblewski GE
American journal of physiology. Gastrointestinal and liver physiology (2013) 3056: G439-52. 110 042 WB; tested species: rat
Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.
Arasaki K, Roy CR
Traffic (Copenhagen, Denmark) (2010) 115: 587-600. 110 042 WB, ICC; tested species: human
Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini.
Falkowski MA, Thomas DD, Groblewski GE
The Journal of biological chemistry (2010) 28546: 35558-66. 110 042 WB
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 110 042 WB, ICC; tested species: rat
Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes.
Liu L, Jedrychowski MP, Gygi SP, Pilch PF
Molecular biology of the cell (2006) 1710: 4249-56. 110 042 WB, ICC; tested species: rat
Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes.
Dolai S, Takahashi T, Qin T, Liang T, Xie L, Kang F, Miao YF, Xie H, Kang Y, Manuel J, Winter E, et al.
Autophagy (2020) : 1-14. 110 042 WB, IP; tested species: human
Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis.
Dolai S, Liang T, Orabi AI, Xie L, Holmyard D, Javed TA, Fernandez NA, Xie H, Cattral MS, Thurmond DC, Thorn P, et al.
The Journal of biological chemistry (2018) 2937: 2510-2522. 110 042 WB, IP; tested species: mouse
Invasion by activated macrophages requires delivery of nascent MT1-MMP through late endosomes/lysosomes to the cell surface.
Röhl J, West ZE, Rudolph M, Zaharia A, Van Lonkhuyzen D, Hickey DK, Semmler ABT, Murray RZ
Traffic (Copenhagen, Denmark) (2019) : . 110 042 WB, ICC; tested species: mouse
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 110 042 WB, ICC
Calpains, cleaved mini-dysferlinC72, and L-type channels underpin calcium-dependent muscle membrane repair.
Lek A, Evesson FJ, Lemckert FA, Redpath GM, Lueders AK, Turnbull L, Whitchurch CB, North KN, Cooper ST
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3312: 5085-94. 110 042 ICC; tested species: human
Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b.
Arasaki K, Roy CR
Traffic (Copenhagen, Denmark) (2010) 115: 587-600. 110 042 WB, ICC; tested species: human
Reduced plasma membrane expression of dysferlin mutants is attributed to accelerated endocytosis via a syntaxin-4-associated pathway.
Evesson FJ, Peat RA, Lek A, Brilot F, Lo HP, Dale RC, Parton RG, North KN, Cooper ST
The Journal of biological chemistry (2010) 28537: 28529-39. 110 042 ICC; tested species: human
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 110 042 WB, ICC; tested species: rat
Role of insulin-dependent cortical fodrin/spectrin remodeling in glucose transporter 4 translocation in rat adipocytes.
Liu L, Jedrychowski MP, Gygi SP, Pilch PF
Molecular biology of the cell (2006) 1710: 4249-56. 110 042 WB, ICC; tested species: rat
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 110 042
Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis.
Li G, Alexander EA, Schwartz JH
The Journal of biological chemistry (2003) 27822: 19791-7. 110 042
From lysosomes to the plasma membrane: localization of vacuolar-type H+ -ATPase with the a3 isoform during osteoclast differentiation.
Toyomura T, Murata Y, Yamamoto A, Oka T, Sun-Wada GH, Wada Y, Futai M
The Journal of biological chemistry (2003) 27824: 22023-30. 110 042
Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules.
Riedel D, Antonin W, Fernandez-Chacon R, Alvarez de Toledo G, Jo T, Geppert M, Valentijn JA, Valentijn K, Jamieson JD, Südhof TC, Jahn R, et al.
Molecular and cellular biology (2002) 2218: 6487-97. 110 042
Background

Syntaxin 4, a member of the SNARE family of proteins, is related to syntaxin 1. Like syntaxin 2 it is predominantly localized to the plasma membrane of a wide variety of cells. Similar to syntaxins 1, 2, and 3, it appears to be involved in the fusion of transport vesicles with the plasma membrane.