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VAChT - 139 105

Acetylcholine transporter in the membrane of synaptic vesicles

Polyclonal Guinea pig purified antibody
450.00 USD
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Cat. No. 139 105 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 475 to 530 from rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: rat (Q62666), mouse (O35304), human (Q16572).
Other species not tested yet.
Specificity Specific for VAChT.
matching control protein/peptide 139-1P
Remarks This antibody is less sensitive compared to the rabbit antibody.VAChT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.
Datasheet 139_105.pdf

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References for VAChT - 139 105

Synaptic FUS Localization During Motoneuron Development and Its Accumulation in Human ALS Synapses.
Deshpande D, Higelin J, Schoen M, Vomhof T, Boeckers TM, Demestre M, Michaelis J
Frontiers in cellular neuroscience (2019) 13: 256. 139 105 ICC; tested species: mouse
Neuregulin 1-ErbB module in C-bouton synapses on somatic motor neurons: molecular compartmentation and response to peripheral nerve injury.
Casanovas A, Salvany S, Lahoz V, Tarabal O, Piedrafita L, Sabater R, Hernández S, Calderó J, Esquerda JE
Scientific reports (2017) 7: 40155. 139 105 ICC
The Val66Met BDNF Polymorphism and Peripheral Nerve Injury: Enhanced Regeneration in Mouse Met-Carriers Is Not Further Improved With Activity-Dependent Treatment.
McGregor CE, Irwin AM, English AW
Neurorehabilitation and neural repair (2019) : 1545968319846131. 139 105 IHC; tested species: mouse
Localization and dynamic changes of neuregulin-1 at C-type synaptic boutons in association with motor neuron injury and repair.
Salvany S, Casanovas A, Tarabal O, Piedrafita L, Hernández S, Santafé M, Soto-Bernardini MC, Calderó J, Schwab MH, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802329R. 139 105 IHC; tested species: mouse
Hypoexcitability precedes denervation in the large fast-contracting motor units in two unrelated mouse models of ALS.
Martinez-Silva ML, Imhoff-Manuel RD, Sharma A, Heckman CJ, Shneider NA, Roselli F, Zytnicki D, Manuel M
eLife (2018) 7: . 139 105 IHC; tested species: mouse
Glial Activation and Central Synapse Loss, but Not Motoneuron Degeneration, Are Prevented by the Sigma-1 Receptor Agonist PRE-084 in the Smn2B/- Mouse Model of Spinal Muscular Atrophy.
Cerveró C, Blasco A, Tarabal O, Casanovas A, Piedrafita L, Navarro X, Esquerda JE, Calderó J
Journal of neuropathology and experimental neurology (2018) : . 139 105 IHC; tested species: mouse
Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.
Case DT, Burton SD, Gedeon JY, Williams SG, Urban NN, Seal RP
Nature communications (2017) 81: 652. 139 105 IHC; tested species: mouse
Neuregulin-1 is concentrated in the postsynaptic subsurface cistern of C-bouton inputs to α-motoneurons and altered during motoneuron diseases.
Gallart-Palau X, Tarabal O, Casanovas A, Sábado J, Correa FJ, Hereu M, Piedrafita L, Calderó J, Esquerda JE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014) 288: 3618-32. 139 105 IHC
Nuclei-specific differences in nerve terminal distribution, morphology, and development in mouse visual thalamus.
Hammer S, Carrillo GL, Govindaiah G, Monavarfeshani A, Bircher JS, Su J, Guido W, Fox MA
Neural development (2014) 9: 16. 139 105 IHC
Altered Dynein Axonemal Assembly Factor 1 Expression in C-Boutons in Bulbar and Spinal Cord Motor-Neurons in Sporadic Amyotrophic Lateral Sclerosis.
Andrés-Benito P, Povedano M, Torres P, Portero-Otín M, Ferrer I
Journal of neuropathology and experimental neurology (2019) : . 139 105 IHC-P; tested species: human
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: | E-Mail: