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SY SY - Synaptic Systems SY SY - Synaptic Systems

VAChT - 139 103

Acetylcholine transporter in the membrane of synaptic vesicles

Polyclonal rabbit purified antibody
370.00 USD
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Cat. No. 139 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 10000 gallery  
IHC: 1 : 100 up to 1 : 1000 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to AA 475 to 530 from rat VAChT (UniProt Id: Q62666)
Reactivity Reacts with: human (Q16572), rat (Q62666), mouse (O35304), pig.
Other species not tested yet.
Specificity Specific for VAChT. (K.O. verified)
matching control protein/peptide 139-1P
Remarks This antibody detects the glycosylated and unglycosylated protein and is an excellent marker for cholinergic axons.

WB: VAChT aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples.

Datasheet 139_103.pdf


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References for VAChT - 139 103

Selective decrease of cholinergic signaling from pedunculopontine and laterodorsal tegmental nuclei has little impact on cognition but markedly increases susceptibility to stress.
Janickova H, Kljakic O, Rosborough K, Raulic S, Matovic S, Gros R, Saksida LM, Bussey TJ, Inoue W, Prado VF, Prado MAM, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802108R. 139 103 WB, IHC; KD verified; tested species: mouse
Altered motor, anxiety-related and attentional task performance at baseline associate with multiple gene copies of the vesicular acetylcholine transporter and related protein overexpression in ChAT::Cre+ rats.
Mantanona CP, Alsiö J, Elson JL, Fisher BM, Dalley JW, Bussey T, Pienaar IS
Brain structure & function (2019) : . 139 103 WB; tested species: rat
Age-related neurochemical and behavioural changes in D409V/WT GBA1 mouse: Relevance to lewy body dementia.
Clarke E, Jantrachotechatchawan C, Buhidma Y, Broadstock M, Yu L, Howlett D, Aarsland D, Ballard C, Francis PT
Neurochemistry international (2019) 129: 104502. 139 103 WB, IHC; tested species: mouse
Cholinergic innervation of principal neurons in the cochlear nucleus of the Mongolian gerbil.
Gillet C, Goyer D, Kurth S, Griebel H, Kuenzel T
The Journal of comparative neurology (2018) 52610: 1647-1661. 139 103 WB, IHC
Deletion of the vesicular acetylcholine transporter from pedunculopontine/laterodorsal tegmental neurons modifies gait.
Janickova H, Rosborough K, Al-Onaizi M, Kljakic O, Guzman MS, Gros R, Prado MA, Prado VF
Journal of neurochemistry (2017) 1405: 787-798. 139 103 WB; tested species: mouse
Cardiac acetylcholine inhibits ventricular remodeling and dysfunction under pathologic conditions.
Roy A, Dakroub M, Tezini GC, Liu Y, Guatimosim S, Feng Q, Salgado HC, Prado VF, Prado MA, Gros R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2016) 302: 688-701. 139 103 WB, IHC
MeCP2 in the enteric nervous system.
Wahba G, Schock SC, Claridge E, Bettolli M, Grynspan D, Humphreys P, Staines WA
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2015) 278: 1156-61. 139 103 WB
An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence.
Frahm S, Antolin-Fontes B, Görlich A, Zander JF, Ahnert-Hilger G, Ibañez-Tallon I
eLife (2015) 4: e11396. 139 103 WB, IHC
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 139 103 WB; tested species: mouse
Severe drug-induced repetitive behaviors and striatal overexpression of VAChT in ChAT-ChR2-EYFP BAC transgenic mice.
Crittenden JR, Lacey CJ, Lee T, Bowden HA, Graybiel AM
Frontiers in neural circuits (2014) 8: 57. 139 103 WB; tested species: mouse
Forebrain deletion of the vesicular acetylcholine transporter results in deficits in executive function, metabolic, and RNA splicing abnormalities in the prefrontal cortex.
Kolisnyk B, Al-Onaizi MA, Hirata PH, Guzman MS, Nikolova S, Barbash S, Soreq H, Bartha R, Prado MA, Prado VF
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3337: 14908-20. 139 103 WB; KO verified; tested species: mouse
ChAT-ChR2-EYFP mice have enhanced motor endurance but show deficits in attention and several additional cognitive domains.
Kolisnyk B, Guzman MS, Raulic S, Fan J, Magalhães AC, Feng G, Gros R, Prado VF, Prado MA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3325: 10427-38. 139 103 WB
Novel strains of mice deficient for the vesicular acetylcholine transporter: insights on transcriptional regulation and control of locomotor behavior.
Martins-Silva C, De Jaeger X, Guzman MS, Lima RD, Santos MS, Kushmerick C, Gomez MV, Caron MG, Prado MA, Prado VF
PloS one (2011) 63: e17611. 139 103 WB, IHC; tested species: mouse
The vesicular acetylcholine transporter is required for neuromuscular development and function.
de Castro BM, De Jaeger X, Martins-Silva C, Lima RD, Amaral E, Menezes C, Lima P, Neves CM, Pires RG, Gould TW, Welch I, et al.
Molecular and cellular biology (2009) 2919: 5238-50. 139 103 WB
Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 139 103 WB; tested species: rat
The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone.
Gras C, Amilhon B, Lepicard EM, Poirel O, Vinatier J, Herbin M, Dumas S, Tzavara ET, Wade MR, Nomikos GG, Hanoun N, et al.
Nature neuroscience (2008) 113: 292-300. 139 103 WB
Immunoisolation of two synaptic vesicle pools from synaptosomes: a proteomics analysis.
Morciano M, Burré J, Corvey C, Karas M, Zimmermann H, Volknandt W
Journal of neurochemistry (2005) 956: 1732-45. 139 103 WB; tested species: rat
Vesicular localization and activity-dependent trafficking of presynaptic choline transporters.
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2330: 9697-709. 139 103 WB, IHC; tested species: mouse
Characterization of projections of longitudinal muscle motor neurons in human colon.
Humenick A, Chen BN, Lauder CIW, Wattchow DA, Zagorodnyuk VP, Dinning PG, Spencer NJ, Costa M, Brookes SJH
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2019) : e13685. 139 103 ICC; tested species: human
Smn-Deficiency Increases the Intrinsic Excitability of Motoneurons.
Arumugam S, Garcera A, Soler RM, Tabares L
Frontiers in cellular neuroscience (2017) 11: 269. 139 103 ICC; tested species: mouse
Catecholaminergic-to-cholinergic transition of sympathetic nerve fibers is stimulated under healthy but not under inflammatory arthritic conditions.
Stangl H, Springorum HR, Muschter D, Grässel S, Straub RH
Brain, behavior, and immunity (2015) 46: 180-91. 139 103 ICC
Minimal Change in the cytoplasmic calcium dynamics in striatal GABAergic neurons of a DYT1 dystonia knock-in mouse model.
Iwabuchi S, Koh JY, Wang K, Ho KW, Harata NC
PloS one (2013) 811: e80793. 139 103 ICC; tested species: mouse
Striatal interneurons in dissociated cell culture.
Schock SC, Jolin-Dahel KS, Schock PC, Staines WA, Garcia-Munoz M, Arbuthnott GW
Histochemistry and cell biology (2010) 1341: 1-12. 139 103 ICC; tested species: mouse,rat
Cellular phenotypes of human model neurons (NT2) after differentiation in aggregate culture.
Podrygajlo G, Tegenge MA, Gierse A, Paquet-Durand F, Tan S, Bicker G, Stern M
Cell and tissue research (2009) 3363: 439-52. 139 103 ICC; tested species: human
A null mutation for the alpha3 nicotinic acetylcholine (ACh) receptor gene abolishes fast synaptic activity in sympathetic ganglia and reveals that ACh output from developing preganglionic terminals is regulated in an activity-dependent retrograde manner.
Rassadi S, Krishnaswamy A, Pié B, McConnell R, Jacob MH, Cooper E
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2537: 8555-66. 139 103 ICC
Human pancreatic afferent and efferent nerves: mapping and 3-D illustration of exocrine, endocrine, and adipose innervation.
Chien HJ, Chiang TC, Peng SJ, Chung MH, Chou YH, Lee CY, Jeng YM, Tien YW, Tang SC
American journal of physiology. Gastrointestinal and liver physiology (2019) : . 139 103 IHC; tested species: human,mouse
Differential expression of VGLUT2 in mouse mesopontine cholinergic neurons.
Steinkellner T, Yoo JH, Hnasko TS
eNeuro (2019) : . 139 103 IHC; tested species: mouse
Age-related neurochemical and behavioural changes in D409V/WT GBA1 mouse: Relevance to lewy body dementia.
Clarke E, Jantrachotechatchawan C, Buhidma Y, Broadstock M, Yu L, Howlett D, Aarsland D, Ballard C, Francis PT
Neurochemistry international (2019) 129: 104502. 139 103 WB, IHC; tested species: mouse
Endogenous Glutamate Excites Myenteric Calbindin Neurons by Activating Group I Metabotropic Glutamate Receptors in the Mouse Colon.
Swaminathan M, Hill-Yardin EL, Bornstein JC, Foong JPP
Frontiers in neuroscience (2019) 13: 426. 139 103 IHC; tested species: mouse
Cell type-specific distribution of GABAA receptor subtypes in the mouse dorsal striatum.
Boccalaro IL, Cristiá-Lara L, Schwerdel C, Fritschy JM, Rubi L
The Journal of comparative neurology (2019) : . 139 103 IHC; tested species: mouse
Selective decrease of cholinergic signaling from pedunculopontine and laterodorsal tegmental nuclei has little impact on cognition but markedly increases susceptibility to stress.
Janickova H, Kljakic O, Rosborough K, Raulic S, Matovic S, Gros R, Saksida LM, Bussey TJ, Inoue W, Prado VF, Prado MAM, et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2019) : fj201802108R. 139 103 WB, IHC; KD verified; tested species: mouse
Cbln2 and Cbln4 are expressed in distinct medial habenula-interpeduncular projections and contribute to different behavioral outputs.
Seigneur E, Polepalli JS, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2018) 11543: E10235-E10244. 139 103 IHC; tested species: mouse
Paracrine Interactions within the Pancreatic Islet Determine the Glycemic Set Point.
Rodriguez-Diaz R, Molano RD, Weitz JR, Abdulreda MH, Berman DM, Leibiger B, Leibiger IB, Kenyon NS, Ricordi C, Pileggi A, Caicedo A, et al.
Cell metabolism (2018) 273: 549-558.e4. 139 103 IHC; tested species: human,mouse
VEGF-A and VEGF-B Coordinate the Arteriogenesis to Repair the Infarcted Heart with Vagus Nerve Stimulation.
Lv YX, Zhong S, Tang H, Luo B, Chen SJ, Chen L, Zheng F, Zhang L, Wang L, Li XY, Yan YW, et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018) 482: 433-449. 139 103 IHC; tested species: rat
Remodeling of cholinergic input to the hippocampus after noise exposure and tinnitus induction in guinea pigs.
Zhang L, Wu C, Martel DT, West M, Sutton MA, Shore SE
Hippocampus (2018) : . 139 103 IHC
Precisely Timed Nicotinic Activation Drives SST Inhibition in Neocortical Circuits.
Urban-Ciecko J, Jouhanneau JS, Myal SE, Poulet JFA, Barth AL
Neuron (2018) 973: 611-625.e5. 139 103 IHC; tested species: mouse
Cholinergic innervation of principal neurons in the cochlear nucleus of the Mongolian gerbil.
Gillet C, Goyer D, Kurth S, Griebel H, Kuenzel T
The Journal of comparative neurology (2018) 52610: 1647-1661. 139 103 WB, IHC
Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.
Case DT, Burton SD, Gedeon JY, Williams SG, Urban NN, Seal RP
Nature communications (2017) 81: 652. 139 103 IHC; tested species: mouse
Serotonin Receptor 5-HT3A Affects Development of Bladder Innervation and Urinary Bladder Function.
Ritter KE, Wang Z, Vezina CM, Bjorling DE, Southard-Smith EM
Frontiers in neuroscience (2017) 11: 690. 139 103 IHC; tested species: mouse
Loss of Sympathetic Nerves in Spleens from Patients with End Stage Sepsis.
Hoover DB, Brown TC, Miller MK, Schweitzer JB, Williams DL
Frontiers in immunology (2017) 8: 1712. 139 103 IHC; tested species: human
Peptidergic nerve fibers in the urethra: Morphological and neurochemical characteristics in female mice of reproductive age.
Barry CM, Ji E, Sharma H, Yap P, Spencer NJ, Matusica D, Haberberger RV
Neurourology and urodynamics (2017) : . 139 103 IHC; tested species: mouse
Differential deregulation of NGF and BDNF neurotrophins in a transgenic rat model of Alzheimer's disease.
Iulita MF, Bistué Millón MB, Pentz R, Aguilar LF, Do Carmo S, Allard S, Michalski B, Wilson EN, Ducatenzeiler A, Bruno MA, Fahnestock M, et al.
Neurobiology of disease (2017) 108: 307-323. 139 103 IHC; tested species: rat
Loss of Neuroligin3 specifically downregulates retinal GABAAα2 receptors without abolishing direction selectivity.
Hoon M, Krishnamoorthy V, Gollisch T, Falkenburger B, Varoqueaux F
PloS one (2017) 127: e0181011. 139 103 IHC; tested species: mouse
Neurturin and a GLP-1 Analogue Act Synergistically to Alleviate Diabetes in Zucker Diabetic Fatty Rats.
Trevaskis JL, Sacramento CB, Jouihan H, Ali S, Le Lay J, Oldham S, Bhagroo N, Boland BB, Cann J, Chang Y, O'Day T, et al.
Diabetes (2017) 667: 2007-2018. 139 103 IHC; tested species: rat
Cardiac acetylcholine inhibits ventricular remodeling and dysfunction under pathologic conditions.
Roy A, Dakroub M, Tezini GC, Liu Y, Guatimosim S, Feng Q, Salgado HC, Prado VF, Prado MA, Gros R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2016) 302: 688-701. 139 103 WB, IHC
Development of early-born γ-Aminobutyric acid hub neurons in mouse hippocampus from embryogenesis to adulthood.
Villette V, Guigue P, Picardo MA, Sousa VH, Leprince E, Lachamp P, Malvache A, Tressard T, Cossart R, Baude A
The Journal of comparative neurology (2016) 52412: 2440-61. 139 103 IHC; tested species: mouse
APOE-Sensitive Cholinergic Sprouting Compensates for Hippocampal Dysfunctions Due to Reduced Entorhinal Input.
Bott JB, Héraud C, Cosquer B, Herbeaux K, Aubert J, Sartori M, Goutagny R, Mathis C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3640: 10472-10486. 139 103 IHC; tested species: mouse
A direct GABAergic output from the basal ganglia to frontal cortex.
Saunders A, Oldenburg IA, Berezovskii VK, Johnson CA, Kingery ND, Elliott HL, Xie T, Gerfen CR, Sabatini BL
Nature (2015) 5217550: 85-9. 139 103 IHC
An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence.
Frahm S, Antolin-Fontes B, Görlich A, Zander JF, Ahnert-Hilger G, Ibañez-Tallon I
eLife (2015) 4: e11396. 139 103 WB, IHC
The effects of caffeine on sleep and maturational markers in the rat.
Olini N, Kurth S, Huber R
PloS one (2013) 89: e72539. 139 103 IHC; tested species: rat
ApoE4 induces synaptic and ERG impairments in the retina of young targeted replacement apoE4 mice.
Antes R, Ezra-Elia R, Weinberger D, Solomon A, Ofri R, Michaelson DM
PloS one (2013) 85: e64949. 139 103 IHC
Tlx3 controls cholinergic transmitter and Peptide phenotypes in a subset of prenatal sympathetic neurons.
Huang T, Hu J, Wang B, Nie Y, Geng J, Cheng L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3326: 10667-75. 139 103 IHC
Optogenetic activation of basal forebrain cholinergic neurons modulates neuronal excitability and sensory responses in the main olfactory bulb.
Ma M, Luo M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3230: 10105-16. 139 103 IHC
A genome-wide screen to identify transcription factors expressed in pelvic Ganglia of the lower urinary tract.
Wiese CB, Ireland S, Fleming NL, Yu J, Valerius MT, Georgas K, Chiu HS, Brennan J, Armstrong J, Little MH, McMahon AP, et al.
Frontiers in neuroscience (2012) 6: 130. 139 103 IHC; tested species: mouse
SMN requirement for synaptic vesicle, active zone and microtubule postnatal organization in motor nerve terminals.
Torres-Benito L, Neher MF, Cano R, Ruiz R, Tabares L
PloS one (2011) 610: e26164. 139 103 IHC; tested species: mouse
Novel strains of mice deficient for the vesicular acetylcholine transporter: insights on transcriptional regulation and control of locomotor behavior.
Martins-Silva C, De Jaeger X, Guzman MS, Lima RD, Santos MS, Kushmerick C, Gomez MV, Caron MG, Prado MA, Prado VF
PloS one (2011) 63: e17611. 139 103 WB, IHC; tested species: mouse
Cholinergic axon length reduced by 300 meters in the brain of an Alzheimer mouse model.
Nikolajsen GN, Jensen MS, West MJ
Neurobiology of aging (2011) 3211: 1927-31. 139 103 IHC
M3 muscarinic acetylcholine receptor expression confers differential cholinergic modulation to neurochemically distinct hippocampal basket cell subtypes.
Cea-del Rio CA, Lawrence JJ, Tricoire L, Erdelyi F, Szabo G, McBain CJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3017: 6011-24. 139 103 IHC
Chronic low-level lead exposure affects the monoaminergic system in the mouse superior olivary complex.
Fortune T, Lurie DI
The Journal of comparative neurology (2009) 5135: 542-58. 139 103 IHC; tested species: mouse
Accumulation of reelin-positive plaques is accompanied by a decline in basal forebrain projection neurons during normal aging.
Madhusudan A, Sidler C, Knuesel I
The European journal of neuroscience (2009) 306: 1064-76. 139 103 IHC
Hibernation model of tau phosphorylation in hamsters: selective vulnerability of cholinergic basal forebrain neurons - implications for Alzheimer's disease.
Härtig W, Stieler J, Boerema AS, Wolf J, Schmidt U, Weissfuss J, Bullmann T, Strijkstra AM, Arendt T
The European journal of neuroscience (2007) 251: 69-80. 139 103 IHC
Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter.
Wang B, Yang L, Wang Z, Zheng H
Proceedings of the National Academy of Sciences of the United States of America (2007) 10435: 14140-5. 139 103 IHC
Mammalian motor neurons corelease glutamate and acetylcholine at central synapses.
Nishimaru H, Restrepo CE, Ryge J, Yanagawa Y, Kiehn O
Proceedings of the National Academy of Sciences of the United States of America (2005) 10214: 5245-9. 139 103 IHC; tested species: mouse
Vesicular localization and activity-dependent trafficking of presynaptic choline transporters.
Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
The Journal of neuroscience : the official journal of the Society for Neuroscience (2003) 2330: 9697-709. 139 103 WB, IHC; tested species: mouse
Evidence for bidirectional and trans-synaptic parasympathetic and sympathetic propagation of alpha-synuclein in rats.
Van Den Berge N, Ferreira N, Gram H, Mikkelsen TW, Alstrup AKO, Casadei N, Tsung-Pin P, Riess O, Nyengaard JR, Tamgüney G, Jensen PH, et al.
Acta neuropathologica (2019) : . 139 103 IHC-P; tested species: rat
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com