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SY SY - Synaptic Systems SY SY - Synaptic Systems

α-Tubulin - 302 203

A major cytoskeleton protein

Polyclonal rabbit purified antibody
370.00 USD
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Cat. No. 302 203 100 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not recommended
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 400 gallery  
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 443 to 449 from rat Δ2-tubulin
Reactivity Reacts with: human, rat, mouse, mammals, chicken.
Other species not tested yet.
Specificity Recognizes glu- and tyr-α-tubulin and Δ2-tubulin.
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 302 211 or 302 201 as capture antibodies (protocol for sandwich-ELISA).

Datasheet 302_203.pdf


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References for alpha-Tubulin - 302 203

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 302 203 WB, ICC, IHC; tested species: mouse,rat
Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy.
Richter KN, Revelo NH, Seitz KJ, Helm MS, Sarkar D, Saleeb RS, D'Este E, Eberle J, Wagner E, Vogl C, Lazaro DF, et al.
The EMBO journal (2018) 371: 139-159. 302 203 ICC; tested species: mouse
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 302 203 WB, ICC, IHC; tested species: mouse,rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 302 203 WB, ICC, IHC; tested species: mouse,rat
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com