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Synaptic vesicle lysate - 504-LP2

Ready to use tissue lysates for western blots
Rat tissue lysate
Cat. No.: 504-LP2
Amount: 50 µg
Price: $280.00
Cat. No. 504-LP2 50 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'.
Total volume: 50 µl. Concentration: 1 mg/ml.
Aliquot and store at -20°C until use.
Applications
 
WB: yes gallery  
Loading/lane 10-30 µg
Source Rat, White Wistar
Remarks

Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.

Data sheet 504-lp2.pdf
Documents MSDS
Cat. No.: 504-LP2
Amount: 50 µg
Price: $280.00
Background

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. Fractions have been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.