Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 119 011BT

SV2 A antibody - 119 011BT

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Mouse monoclonal purified IgG
Cat. No.: 119 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 119 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks)
IP: yes
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label biotin
Clone 17G10
Subtype IgG3
Immunogen Synthetic peptide corresponding to AA 2 to 17 from human SV2A (UniProt Id: Q7L0J3)
Reactivity Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5).
Other species not tested yet.
Matching control protein/peptide 119-0P
Remarks

WB:  SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 119_011bt.pdf
Cat. No.: 119 011BT
Amount: 100 µg
Price: $465.00
ICC
Background
SV 2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV 2A, SV 2B and SV 2C. SV 2A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV 2B has a more restricted distribution with varying degrees of coexpression with SV 2A. SV 2C is more closely related to SV 2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.
Other products for this target